Co-cultivo de neuronas y microglía tratadas con neuromelanina como modelo de estudio de los procesos involucrados en la agregación de proteínas en la enfermedad del Parkinson

Matute Lamana, Carlos
Carrodeguas Villar, José Alberto (dir.) ; De la Fuente Herreruela, Diego (dir.)

Universidad de Zaragoza, CIEN, 2021
Bioquímica y Biología Molecular y Celular department, Bioquímica y Biología Molecular area

Máster Universitario en Biotecnología Cuantitativa

Abstract: Parkinson's disease is a common neurodegenerative disease that affects more than 10 million people worldwide which causes motor abnormalities such as tremor, muscle stiffness, paucity of voluntary movements and postural instability. It is mainly characterized by neural degeneration in the substantia nigra of the mid brain. Misfolding and aggregation of alpha-synuclein (aSyn) is one of the major molecular hallmarks of the disease but it is not clear which are the initial molecular and cellular mechanisms leading to protein aggregation, although cellular stress caused by oxidative damage with the involvement of neuromelanin and microglia-mediated inflammatory responses appear to be involved. No experimental model has been able so far to accurately replicate the situation within the human brain. In this work, we have used co-cultures of in vitro-differentiated dopaminergic neurons (SH-SY5Y) together with an immortalized human microglia cell line (HMC3), treated with synthetic neuromelanin, to approach the initial processes leading to aSyn aggregation using 2D models.

Tipo de Trabajo Académico: Trabajo Fin de Master