000108653 001__ 108653 000108653 005__ 20211216104750.0 000108653 037__ $$aTAZ-TFM-2021-523 000108653 041__ $$aeng 000108653 1001_ $$aJayakumar, Sharath 000108653 24200 $$aModification of membranes surfaces for blood protein separation 000108653 24500 $$aModification of membranes surfaces for blood protein separation 000108653 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2021 000108653 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000108653 520__ $$aThe main idea behind this project is to separate platelet derived growth factor (PDGF) from platelets which has a promising role to play for medical applications such as treating of diabetic ulcers and chemotherapy. The present techniques used to separate PDGF consumes large amount of energy, and the yield is also poor. Hence an alternate method of separating PDGF from platelets is by membrane separation. The main objective of this project is to modify the membrane surface with a block copolymer that can selectively capture platelets by avoiding unwanted interactions with other blood components. The present study will focus on this first step of coating the membrane and evaluation of unwanted adsorption of blood proteins. Various optimization conditions like coating time, concentration of copolymer, chain length and operating process were varied, and the coating was analyzed using FTIR mapping. Coating time of 2 hours and the copolymer concentration of 5 mg/mL following the immersion, washing, drying (IWD) process were the optimum conditions. Filtration was done over the coated membranes and the permeability was found to decrease with increase in size of the copolymer. Then filtration was done with protein solution and the concentration of protein in retentate and permeate was calculated followed by retention %. FTIR mapping was done on filtration membrane to check if there was removal of coating from the membrane surface. From the mapping it was clear that the coating was present on the membrane surface even after filtration. For future studies, it is recommended to use other blood proteins like globulin and fibrinogen on the adhesion with the copolymer. Other characterization tools like SEM and AFM can be used to analyze the morphology and surface roughness of the coated membrane, respectively. <br /><br /> 000108653 521__ $$aMáster Universitario Erasmus Mundus en Ingeniería de Membranas 000108653 540__ $$aDerechos regulados por licencia Creative Commons 000108653 700__ $$aCausserand, Christel$$edir. 000108653 7102_ $$aUniversidad de Zaragoza$$b $$c 000108653 8560_ $$f836091@unizar.es 000108653 8564_ $$s1708246$$uhttps://zaguan.unizar.es/record/108653/files/TAZ-TFM-2021-523.pdf$$yMemoria (eng) 000108653 909CO $$ooai:zaguan.unizar.es:108653$$pdriver$$ptrabajos-fin-master 000108653 950__ $$a 000108653 951__ $$adeposita:2021-12-16 000108653 980__ $$aTAZ$$bTFM$$cCIEN 000108653 999__ $$a20210628124249.CREATION_DATE