000108668 001__ 108668 000108668 005__ 20211216104751.0 000108668 037__ $$aTAZ-TFM-2021-479 000108668 041__ $$aeng 000108668 1001_ $$aDotor Delgado, Laura 000108668 24200 $$aMimic Cell Membranes: thermodynamic and atomic force microscopy studies 000108668 24500 $$aModelos de membrana celular: estudio termodinámico y microscopía de fuerza atómica 000108668 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2021 000108668 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/ 000108668 520__ $$aMimic cell membranes are crucial for the understanding of cellular processes since the biological membrane is too complex to be deeply studied in vivo. In this Master’s Project, dipalmitoylphosphatidylcholine (DPPC) and cholesterol (CHOL) constitute the model cell membrane in order to study their interaction with curcumin (CCM). In the last decades, this xenobiotic has become a relevant compound in encapsulated cell therapy and clinical trials because it exhibits anti-cancer, antioxidant, neuroprotector, antidepressant and anti-inflammatory effects. Consequently, a comprehensive study at the air-water interface of single, binary and ternary monolayers was performed by means of the Langmuir technique. The thermodynamic results are indicative of a partial miscibility between the components. In general, the miscibility increases as the monolayer is more condensed and, depending on the composition, the monolayers show more expanded phases as compared to those of the pure compounds. For both CCM-DPPC and CCM-CHOL binary monolayers, the most favourable mixture in terms of the steric and energetic effects is xCCM = 0.8. On the other hand, for the DPPC-CHOL binary monolayers, the most favourable mixture is xDPPC = 0.6. With respect to the ternary monolayers, the most favourable mixtures are xCCM = 0.2 and 0.4; it must be taken into account that the Eucaryotic cells contain DPPC-CHOL 1:1 and that the biological surface pressure is about 30 mN/m. Finally, selected Langmuir-Blodgett films were inspected by Atomic Force Microscopy (AFM); the morphology of the images supports the conclusion of partial miscibility of the components as deduced from the thermodynamic study.<br /><br /> 000108668 521__ $$aMáster en Materiales Nanoestructurados para Aplicaciones Nanotecnológicas (Nanostructured Materials for Nanotechnology Applications) 000108668 540__ $$aDerechos regulados por licencia Creative Commons 000108668 700__ $$aCea Mingueza, Pilar$$edir. 000108668 700__ $$aMartín Solans, Santiago$$edir. 000108668 7102_ $$aUniversidad de Zaragoza$$bQuímica Física$$cQuímica Física 000108668 8560_ $$f738269@unizar.es 000108668 8564_ $$s5755410$$uhttps://zaguan.unizar.es/record/108668/files/TAZ-TFM-2021-479.pdf$$yMemoria (eng) 000108668 909CO $$ooai:zaguan.unizar.es:108668$$pdriver$$ptrabajos-fin-master 000108668 950__ $$a 000108668 951__ $$adeposita:2021-12-16 000108668 980__ $$aTAZ$$bTFM$$cCIEN 000108668 999__ $$a20210628092816.CREATION_DATE