CASA-Mot technology: how results are affected by the frame rate and counting chamber

Bompart, D. ; Garcia-Molina, A. ; Valverde, A. ; Caldeira, C. ; Yaniz, J. (Universidad de Zaragoza) ; Núñez de Murga, M. ; Soler, C.

Resumen: For over 30 years, CASA-Mot technology has been used for kinematic analysis of sperm motility in different mammalian species, but insufficient attention has been paid to the technical limitations of commercial computer-aided sperm analysis (CASA) systems. Counting chamber type and frame rate are two of the most important aspects to be taken into account. Counting chambers can be disposable or reusable, with different depths. In human semen analysis, reusable chambers with a depth of 10 mm are the most frequently used, whereas for most farm animal species it is more common to use disposable chambers with a depth of 20 mm. The frame rate was previously limited by the hardware, although changes in the number of images collected could lead to significant variations in some kinematic parameters, mainly in curvilinear velocity (VCL). A frame rate of 60 frames s(-1) is widely considered to be the minimum necessary for satisfactory results. However, the frame rate is species specific and must be defined in each experimental condition. In conclusion, we show that the optimal combination of frame rate and counting chamber type and depth should be defined for each species and experimental condition in order to obtain reliable results.

Idioma: Inglés

DOI: 10.1071/RD17551

Año: 2018

Publicado en: REPRODUCTION FERTILITY AND DEVELOPMENT 30, 6 (2018), 810-819
ISSN: 1031-3613

Factor impacto JCR: 1.723 (2018)

Categ. JCR: ZOOLOGY rank: 42 / 170 = 0.247 (2018) - Q1 - T1
Categ. JCR: DEVELOPMENTAL BIOLOGY rank: 31 / 42 = 0.738 (2018) - Q3 - T3
Categ. JCR: REPRODUCTIVE BIOLOGY rank: 24 / 29 = 0.828 (2018) - Q4 - T3

Factor impacto SCIMAGO: 0.716 - Animal Science and Zoology (Q1) - Biotechnology (Q1) - Developmental Biology (Q1) - Reproductive Medicine (Q1) - Genetics (Q1) - Molecular Biology (Q1) - Endocrinology (Q1)

Financiación: info:eu-repo/grantAgreement/EC/H2020/642893/EU/Improved production strategies for endangered freshwater species/IMPRESS
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P

Tipo y forma: Revisión (Versión definitiva)

Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)