Quantification of Lipoprotein Uptake in Vivo Using Magnetic Particle Imaging and Spectroscopy

Hildebrand, S. ; Löwa, N. ; Paysen, H. ; Fratila, R.M. ; Reverte-Salisa, L. ; Trakoolwilaiwan, T. ; Niu, Z. ; Kasparis, G. ; Preuss, S.F. ; Kosch, O. ; De La Fuente, J.M. ; Thanh, N.T.K. ; Wiekhorst, F. ; Pfeifer, A.

Resumen: Lipids are a major source of energy for most tissues, and lipid uptake and storage is therefore crucial for energy homeostasis. So far, quantification of lipid uptake in vivo has primarily relied on radioactive isotope labeling, exposing human subjects or experimental animals to ionizing radiation. Here, we describe the quantification of in vivo uptake of chylomicrons, the primary carriers of dietary lipids, in metabolically active tissues using magnetic particle imaging (MPI) and magnetic particle spectroscopy (MPS). We show that loading artificial chylomicrons (ACM) with iron oxide nanoparticles (IONPs) enables rapid and highly sensitive post hoc detection of lipid uptake in situ using MPS. Importantly, by utilizing highly magnetic Zn-doped iron oxide nanoparticles (ZnMNPs), we generated ACM with MPI tracer properties superseding the current gold-standard, Resovist, enabling quantification of lipid uptake from whole-animal scans. We focused on brown adipose tissue (BAT), which dissipates heat and can consume a large part of nutrient lipids, as a model for tightly regulated and inducible lipid uptake. High BAT activity in humans correlates with leanness and improved cardiometabolic health. However, the lack of nonradioactive imaging techniques is an important hurdle for the development of BAT-centered therapies for metabolic diseases such as obesity and type 2 diabetes. Comparison of MPI measurements with iron quantification by inductively coupled plasma mass spectrometry revealed that MPI rivals the performance of this highly sensitive technique. Our results represent radioactivity-free quantification of lipid uptake in metabolically active tissues such as BAT.

Idioma: Inglés

DOI: 10.1021/acsnano.0c03229

Año: 2021

Publicado en: ACS NANO 15 (2021), 434-446
ISSN: 1936-0851

Factor impacto JCR: 18.027 (2021)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 13 / 180 = 0.072 (2021) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 20 / 345 = 0.058 (2021) - Q1 - T1
Categ. JCR: CHEMISTRY, PHYSICAL rank: 12 / 165 = 0.073 (2021) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 11 / 109 = 0.101 (2021) - Q1 - T1

Factor impacto CITESCORE: 24.3 - Engineering (Q1) - Physics and Astronomy (Q1) - Materials Science (Q1)

Factor impacto SCIMAGO: 4.611 - Engineering (miscellaneous) (Q1) - Physics and Astronomy (miscellaneous) (Q1) - Materials Science (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/E15-17R
Financiación: info:eu-repo/grantAgreement/EC/H2020/657215/EU/Cell-surface immobilized vs. internalized magnetic nanoparticles for magnetic hyperthermia studies/OUTstandINg
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2017-84246-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RYC-2015-17640

Tipo y forma: Artículo (PostPrint)