000109066 001__ 109066 000109066 005__ 20230519145445.0 000109066 0247_ $$2doi$$a10.1128/AAC.01693-20 000109066 0248_ $$2sideral$$a122408 000109066 037__ $$aART-2021-122408 000109066 041__ $$aeng 000109066 100__ $$aSerrano-Lobo, J. 000109066 245__ $$aAzole and amphotericin B MIC values against aspergillus fumigatus: High agreement between spectrophotometric and visual readings using the EUCAST EDef 9.3.2 procedure 000109066 260__ $$c2021 000109066 5060_ $$aAccess copy available to the general public$$fUnrestricted 000109066 5203_ $$aThe EUCAST EDef 9.3.2 procedure recommends visual readings of azole and amphotericin B MICs against Aspergillus spp. Visual determination of MICs may be challenging. In this work, we aim to obtain and compare visual and spectrophotometric MIC readings of azoles and amphotericin B against Aspergillus fumigatus sensu lato isolates. A total of 847 A. fumigatus sensu lato isolates (A. fumigatus sensu stricto [n = 828] and cryptic species [n = 19]) were tested against amphotericin B, itraconazole, voriconazole, posaconazole, and isavuconazole using the EUCAST EDef 9.3.2 procedure. Isolates were classified as susceptible or resistant/non-wild type according to the 2020 updated breakpoints. The area of technical uncertainty for the azoles was defined in the updated breakpoints. Visual and spectrophotometric (fungal growth reduction of >95% compared to the control, read at 540 nm) MICs were compared. Essential (+1 2-fold dilution) and categorical agreements were calculated. Overall, high essential (97.1%) and categorical (99.6%) agreements were found. We obtained 100% categorical agreements for amphotericin B, itraconazole, and posaconazole, and consequently, no errors were found. Categorical agreements were 98.7 and 99.3% for voriconazole and isavuconazole, respectively. Most of the misclassifications for voriconazole and isavuconazole were found to be associated with MIC results falling either in the area of technical uncertainty or within one 2-fold dilution above the breakpoint. The resistance rate was slightly lower when the MICs were obtained by spectrophotometric readings. However, all relevant cyp51A mutants were correctly classified as resistant. Spectrophotometric determination of azole and amphotericin B MICs against A. fumigatus sensu lato isolates may be a convenient alternative to visual endpoint readings. 000109066 536__ $$9info:eu-repo/grantAgreement/EUR/ISCII-ERDF/A way to make Europe$$9info:eu-repo/grantAgreement/ES/ISCIII-FIS/CP15-00115 000109066 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000109066 590__ $$a5.938$$b2021 000109066 592__ $$a1.546$$b2021 000109066 594__ $$a9.3$$b2021 000109066 591__ $$aPHARMACOLOGY & PHARMACY$$b51 / 279 = 0.183$$c2021$$dQ1$$eT1 000109066 593__ $$aPharmacology$$c2021$$dQ1 000109066 591__ $$aMICROBIOLOGY$$b35 / 138 = 0.254$$c2021$$dQ2$$eT1 000109066 593__ $$aInfectious Diseases$$c2021$$dQ1 000109066 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000109066 700__ $$aGómez, A. 000109066 700__ $$aSánchez-Yebra, W. 000109066 700__ $$aFajardo, M. 000109066 700__ $$aLorenzo, B. 000109066 700__ $$aSánchez-Reus, F. 000109066 700__ $$aVidal, I. 000109066 700__ $$aFernández-Torres, M. 000109066 700__ $$aSánchez-Romero, I. 000109066 700__ $$ade Alegría-Puig, C.R. 000109066 700__ $$adel Pozo, J.L. 000109066 700__ $$aMuñoz, P. 000109066 700__ $$aEscribano, P. 000109066 700__ $$aGuinea, J. 000109066 700__ $$aSánchez-Gómez, J. 000109066 700__ $$aLozano, I. 000109066 700__ $$aMarfil, E. 000109066 700__ $$ade la Rosa, M.M. 000109066 700__ $$aGarcía, R.T. 000109066 700__ $$aCobo, F. 000109066 700__ $$aCastro, C. 000109066 700__ $$0(orcid)0000-0002-6227-861X$$aLópez, C.$$uUniversidad de Zaragoza 000109066 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza 000109066 700__ $$aPeláez, T. 000109066 700__ $$aCastelló-Abietar, C. 000109066 700__ $$aCos-Tales, I. 000109066 700__ $$aSerra, J.L. 000109066 700__ $$aJiménez, R. 000109066 700__ $$aEcheverría, C.L. 000109066 700__ $$aPérez, C.L. 000109066 700__ $$aMegías-Lobón, G. 000109066 700__ $$aAyats, J. 000109066 700__ $$aMartín, M.T. 000109066 700__ $$aSánchez-Hellín, V. 000109066 700__ $$aIbáñez, E. 000109066 700__ $$aPemán, J. 000109066 700__ $$aPazos, C. 000109066 700__ $$aRodríguez-Mayo, M. 000109066 700__ $$aPérez-Ayala, A. 000109066 700__ $$aGómez, E. 000109066 700__ $$aSerrano, J. 000109066 700__ $$aReigadas, E. 000109066 700__ $$aRodríguez, B. 000109066 700__ $$aZvezdanova, E. 000109066 700__ $$aDíaz-García, J. 000109066 700__ $$aGonzález Leiva, J. 000109066 700__ $$aMachado, M. 000109066 700__ $$aGarcía-Rodríguez, J. 000109066 700__ $$aVallejo, M.R. 000109066 700__ $$aLópez-Soria, L. 000109066 700__ $$aMarimón, J.M. 000109066 700__ $$aVicente, D. 000109066 700__ $$aHernáez-Crespo, S. 000109066 7102_ $$15011$$2220$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Ecología 000109066 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología 000109066 773__ $$g65, 1 (2021), [28 pp]$$pAntimicrob. agents chemother.$$tAntimicrobial Agents and Chemotherapy$$x0066-4804 000109066 8564_ $$s1374896$$uhttps://zaguan.unizar.es/record/109066/files/texto_completo.pdf$$yPostprint 000109066 8564_ $$s1608102$$uhttps://zaguan.unizar.es/record/109066/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000109066 909CO $$ooai:zaguan.unizar.es:109066$$particulos$$pdriver 000109066 951__ $$a2023-05-18-14:37:25 000109066 980__ $$aARTICLE