000109073 001__ 109073
000109073 005__ 20230519145446.0
000109073 0247_ $$2doi$$a10.3390/f12121634
000109073 0248_ $$2sideral$$a125484
000109073 037__ $$aART-2021-125484
000109073 041__ $$aeng
000109073 100__ $$aMartínez, Mª Teresa
000109073 245__ $$aMicropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees
000109073 260__ $$c2021
000109073 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109073 5203_ $$aHolm oak populations have deteriorated drastically due to oak decline syndrome. The first objective of the present study was to investigate the use of axillary budding and somatic embryogenesis (SE) to propagate asymptomatic holm oak genotypes identified in disease hotspots in Spain. Axillary budding was achieved in two out of six tolerant genotypes from the south-western region and in two out of four genotypes from the Mediterranean region. Rooting of shoots cultured on medium supplemented with 3 mg L\u22121 of indole-3-acetic acid plus 0.1 mg L\u22121 a-naphthalene acetic acid was achieved, with rates ranging from 8 to 36%. Shoot cultures remained viable after cold storage for 9-12 months; this procedure is therefore suitable for medium-term conservation of holm oak germplasm. SE was induced in two out of the three genotypes tested, by using nodes and shoot tips cultured in medium without plant growth regulators. In vitro cloned progenies of the tolerant genotypes PL-T2 and VA5 inhibited growth of Phytophthora cinnamomi mycelia when exposed to the oomycete in vitro. Significant differences in total phenol contents and in the expression profiles of genes regulating phenylpropanoid biosynthesis were observed between in vitro cultured shoots derived from tolerant trees and cultures established from control genotypes.
000109073 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109073 590__ $$a3.282$$b2021
000109073 592__ $$a0.623$$b2021
000109073 594__ $$a4.0$$b2021
000109073 591__ $$aFORESTRY$$b14 / 70 = 0.2$$c2021$$dQ1$$eT1
000109073 593__ $$aForestry$$c2021$$dQ1
000109073 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109073 700__ $$aArrillaga, Isabel
000109073 700__ $$0(orcid)0000-0003-0582-5418$$aSales, Ester$$uUniversidad de Zaragoza
000109073 700__ $$aPérez-Oliver, María Amparo
000109073 700__ $$aGonzález-Mas, Mª del Carmen
000109073 700__ $$aCorredoira, Elena
000109073 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000109073 773__ $$g12 (2021), 1634 [23 pp]$$pForests$$tForests$$x1999-4907
000109073 8564_ $$s979167$$uhttps://zaguan.unizar.es/record/109073/files/texto_completo.pdf$$yVersión publicada
000109073 8564_ $$s2841955$$uhttps://zaguan.unizar.es/record/109073/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109073 909CO $$ooai:zaguan.unizar.es:109073$$particulos$$pdriver
000109073 951__ $$a2023-05-18-14:38:06
000109073 980__ $$aARTICLE