000109128 001__ 109128
000109128 005__ 20240124152521.0
000109128 0247_ $$2doi$$a10.1021/acsbiomaterials.0c01382
000109128 0248_ $$2sideral$$a122387
000109128 037__ $$aART-2021-122387
000109128 041__ $$aeng
000109128 100__ $$aVirumbrales-Muñoz, María
000109128 245__ $$aForce spectroscopy imaging and constriction assays reveal the effects of graphene oxide on the mechanical properties of alginate microcapsules
000109128 260__ $$c2021
000109128 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109128 5203_ $$aMicroencapsulation of cells in hydrogel-based porous matrices is an approach that has demonstrated great success in regenerative cell therapy. These microcapsules work by concealing the exogenous cells and materials in a robust biomaterial that prevents their recognition by the immune system. A vast number of formulations and additives are continuously being tested to optimize cell viability and mechanical properties of the hydrogel. Determining the effects of new microcapsule additives is a lengthy process that usually requires extensive in vitro and in vivo testing. In this paper, we developed a workflow using nanoindentation (i.e., indentation with a nanoprobe in an atomic force microscope) and a custom-built microfluidic constriction device to characterize the effect of graphene oxide (GO) on three microcapsule formulations. With our workflow, we determined that GO modifies the microcapsule stiffness and surface properties in a formulation-dependent manner. Our results also suggest, for the first time, that GO alters the conformation of the microcapsule hydrogel and its interaction with subsequent coatings. Overall, our workflow can infer the effects of new additives on microcapsule surfaces. Thus, our workflow can contribute to diminishing the time required for the validation of new microcapsule formulations and accelerate their clinical translation.
000109128 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T62-20R$$9info:eu-repo/grantAgreement/EC/H2020/78132/EU/Electronic Components and Systems for European Leadership Joint Undertaking/ECSEL JU-78132-Position-II-2017-IA$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 78132-ECSEL JU-78132-Position-II-2017-IA$$9info:eu-repo/grantAgreement/ES/MEC/FPU12-05640$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/PGC2018-097257-B-C31
000109128 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-sa$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/es/
000109128 590__ $$a5.395$$b2021
000109128 592__ $$a0.935$$b2021
000109128 594__ $$a8.3$$b2021
000109128 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b18 / 45 = 0.4$$c2021$$dQ2$$eT2
000109128 593__ $$aBiomedical Engineering$$c2021$$dQ1
000109128 593__ $$aBiomaterials$$c2021$$dQ1
000109128 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000109128 700__ $$0(orcid)0000-0001-6139-5905$$aPaz-Artigas, Laura$$uUniversidad de Zaragoza
000109128 700__ $$0(orcid)0000-0002-8666-622X$$aCiriza, Jesús$$uUniversidad de Zaragoza
000109128 700__ $$0(orcid)0000-0002-7854-8856$$aAlcaine, Clara$$uUniversidad de Zaragoza
000109128 700__ $$aEspona-Noguera, Albert
000109128 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000109128 700__ $$aSáenz del Burgo, Laura
000109128 700__ $$aZiani, Kaoutar
000109128 700__ $$aPedraz, José Luis
000109128 700__ $$aFernández, Luis
000109128 700__ $$aOchoa, Ignacio
000109128 7102_ $$11006$$2255$$aUniversidad de Zaragoza$$bDpto. Fisiatría y Enfermería$$cÁrea Enfermería
000109128 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000109128 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000109128 773__ $$g7 (2021), 242-253$$pACS biomater. sci. eng.$$tACS BIOMATERIALS SCIENCE & ENGINEERING$$x2373-9878
000109128 8564_ $$s735817$$uhttps://zaguan.unizar.es/record/109128/files/texto_completo.pdf$$yPostprint
000109128 8564_ $$s2939923$$uhttps://zaguan.unizar.es/record/109128/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000109128 909CO $$ooai:zaguan.unizar.es:109128$$particulos$$pdriver
000109128 951__ $$a2024-01-24-15:17:24
000109128 980__ $$aARTICLE