000109345 001__ 109345
000109345 005__ 20220111162036.0
000109345 0247_ $$2doi$$a10.1177/0300985820980711
000109345 0248_ $$2sideral$$a122718
000109345 037__ $$aART-2020-122718
000109345 041__ $$aeng
000109345 100__ $$0(orcid)0000-0003-3792-287X$$aDe las Heras, M.$$uUniversidad de Zaragoza
000109345 245__ $$aExogenous Small Ruminant Betaretrovirus Envelope Protein Is Detected in Draining Lymph Nodes in Contagious Respiratory Tumors of Sheep and Goats
000109345 260__ $$c2020
000109345 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109345 5203_ $$aContagious respiratory tumors of sheep and goats are epithelial neoplasms of the lung and nasal cavities. They are associated with oncogenic betaretroviruses known as jaagsiekte sheep retrovirus and enzootic nasal tumor retrovirus of sheep and goats. We investigated the presence of the envelope protein (ENV) of these retroviruses in retropharyngeal and mediastinal lymph nodes using a specific monoclonal antibody by immunohistochemistry methods, single-labeled or combined with ovine B or T lymphocytes or macrophage cell markers. Samples of lymph nodes, fixed in formalin and zinc fixative, were obtained from paraffin-embedded material. Four groups of samples were used: 24 natural cases of ovine pulmonary adenocarcinoma (OPA), 13 of enzootic nasal adenocarcinoma of sheep (ENAS), 19 of enzootic nasal adenocarcinoma of goats (ENAG), and 14 control samples. ENV was detected by single labeling in cortical lymphoid follicles. Six of 24 OPA samples were positive and only in those from sheep with extensive neoplasia. Immunolabeling was detected in 5/13 ENAS and 10/19 ENAG samples. Positive labeling was found either in the intercellular spaces, membranes, or cytoplasm of cells in follicles. Control samples were not correspondingly labeled. Double immunohistochemistry demonstrated co-labeling of ENV and CD21 (B cells and follicular dendritic cells) in all samples, CD14 (macrophage) in OPA samples, and Pax-5 (B cells) in ENAG samples, but not with CD8 or CD4 (T lymphocytes). These results demonstrate the presence of betaretrovirus ENV proteins in nontumor cells in regional lymph nodes in sheep and goats with contagious respiratory tumors.
000109345 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/Construyendo Aragón 2016–2020-A15-17R$$9info:eu-repo/grantAgreement/ES/UZ/Research group-Welfare and pathology of small ruminants
000109345 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000109345 590__ $$a2.221$$b2020
000109345 591__ $$aVETERINARY SCIENCES$$b40 / 146 = 0.274$$c2020$$dQ2$$eT1
000109345 591__ $$aPATHOLOGY$$b47 / 77 = 0.61$$c2020$$dQ3$$eT2
000109345 592__ $$a0.794$$b2020
000109345 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1
000109345 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000109345 700__ $$aReséndiz, R.A.
000109345 700__ $$0(orcid)0000-0002-4655-9171$$aGonzález-Sáinz, J.M.$$uUniversidad de Zaragoza
000109345 700__ $$0(orcid)0000-0002-0313-5926$$aOrtín, A.$$uUniversidad de Zaragoza
000109345 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000109345 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000109345 773__ $$g(2020), 1-8$$pVet. pathol.$$tVeterinary Pathology$$x0300-9858
000109345 8564_ $$s369931$$uhttps://zaguan.unizar.es/record/109345/files/texto_completo.pdf$$yPostprint
000109345 8564_ $$s2525065$$uhttps://zaguan.unizar.es/record/109345/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000109345 909CO $$ooai:zaguan.unizar.es:109345$$particulos$$pdriver
000109345 951__ $$a2022-01-11-13:45:56
000109345 980__ $$aARTICLE