000109395 001__ 109395
000109395 005__ 20231215090957.0
000109395 0247_ $$2doi$$a10.1016/j.foodres.2021.110403
000109395 0248_ $$2sideral$$a125445
000109395 037__ $$aART-2021-125445
000109395 041__ $$aeng
000109395 100__ $$0(orcid)0000-0001-5053-8309$$aBerdejo, Daniel$$uUniversidad de Zaragoza
000109395 245__ $$aEmerging mutant populations of Listeria monocytogenes EGD-e under selective pressure of Thymbra capitata essential oil question its use in food preservation
000109395 260__ $$c2021
000109395 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109395 5203_ $$aDue to their excellent antimicrobial properties, essential oils (EO) have been proposed as potential preservatives for certain kinds of foods, such as dairy products. However, the occurrence of pathogenic populations that are resistant to EOs could pose a health risk. This report seeks to assess the emergence of resistant populations in Listeria monocytogenes EGD-e growth at 37 °C under selective pressure of Thymbra capitata EO (TCO), to characterise their resistance in laboratory media, and to identify their genotypic changes, as well as to evaluate the resistance in skimmed milk. TCO cyclic treatment allowed the isolation of two L. monocytogenes EGD-e resistant strains against the EO: LmSTCO by sublethal doses (75 µL/L TCO) and LmLTCO by lethal doses (300 µL/L TCO) after 20 and 30 cycles, respectively. Both strains displayed an increase of the minimum inhibitory and bactericidal concentration against TCO and a higher survival rate after lethal treatments than the wild-type strain (LmWT). Growth kinetics revealed a better adaptation of LmSTCO in presence of TCO, while LmLTCO grew more slowly compared to LmWT, even in the absence of the antimicrobial. Moreover, a slight increase in cross-resistance to antibiotics was observed: LmSTCO to β-lactams and LmLTCO to a series of broad-spectrum antibiotics. The genomic study revealed one sole nucleotide change in LmSTCO located in plsC gene codifying an enzyme involved in the production of phosphatidic acid, a precursor in cell membrane synthesis. Five genetic variations were found in LmLTCO: among them, the deletion of an ATP-synthesis system involved in slowing bacterial growth. Inhibition and inactivation assays in skimmed milk confirmed the increased resistance of both strains, thereby indicating a safety risk in case these strains emerge in the food chain. These results strongly suggest that the occurrence of such resistances should be taken into account in order to ensure the efficacy of natural antimicrobials in the design of food preservation strategies.
000109395 536__ $$9info:eu-repo/grantAgreement/ES/AEI-ESF-DGA/PGC2018-093789-B-I00
000109395 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109395 590__ $$a7.425$$b2021
000109395 592__ $$a1.248$$b2021
000109395 594__ $$a11.1$$b2021
000109395 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b13 / 144 = 0.09$$c2021$$dQ1$$eT1
000109395 593__ $$aFood Science$$c2021$$dQ1
000109395 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109395 700__ $$0(orcid)0000-0003-3752-4660$$aPagán, Elisa$$uUniversidad de Zaragoza
000109395 700__ $$0(orcid)0000-0003-4460-3543$$aMerino, Natalia$$uUniversidad de Zaragoza
000109395 700__ $$0(orcid)0000-0002-7629-8101$$aGarcía-Gonzalo, Diego$$uUniversidad de Zaragoza
000109395 700__ $$0(orcid)0000-0002-0238-6328$$aPagán, Rafael$$uUniversidad de Zaragoza
000109395 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000109395 773__ $$g145 (2021), 110403 [9 pp.]$$pFood res. int.$$tFood Research International$$x0963-9969
000109395 8564_ $$s1326997$$uhttps://zaguan.unizar.es/record/109395/files/texto_completo.pdf$$yVersión publicada
000109395 8564_ $$s2577707$$uhttps://zaguan.unizar.es/record/109395/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109395 909CO $$ooai:zaguan.unizar.es:109395$$particulos$$pdriver
000109395 951__ $$a2023-12-15-09:00:31
000109395 980__ $$aARTICLE