109410 20240319080948.0 doi 10.1115/1.4053143 sideral 125420 ART-2022-125420 eng Juste-Lanas, Yago Universidad de Zaragoza (orcid)0000-0003-2237-8859 Confined cell migration and asymmetric hydraulic environments to evaluate the metastatic potential of cancer cells 2022 Access copy available to the general public Unrestricted Metastasis, a hallmark of cancer development, is also the leading reason for most cancer-related deaths. Furthermore, cancer cells are highly adaptable to microenvironments and can migrate along pre-existing channel-like tracks of anatomical structures. However, more representative three-dimensional models are required to reproduce the heterogeneity of metastatic cell migration in vivo to further understand the metastasis mechanism and develop novel therapeutic strategies against it. Here, we designed and fabricated different microfluidic-based devices that recreate confined migration and diverse environments with asymmetric hydraulic resistances. Our results show different migratory potential between metastatic and nonmetastatic cancer cells in confined environments. Moreover, although nonmetastatic cells have not been tested against barotaxis due to their low migration capacity, metastatic cells present an enhanced preference to migrate through the lowest resistance path, being sensitive to barotaxis. This device, approaching the study of metastasis capability based on confined cell migration and barotactic cell decisions, may pave the way for the implementation of such technology to determine and screen the metastatic potential of certain cancer cells. info:eu-repo/grantAgreement/ES/DGA-FEDER/RIS3-LMP74-18 info:eu-repo/grantAgreement/EC/H2020/826494/EU/PRedictive In-silico Multiscale Analytics to support cancer personalized diaGnosis and prognosis, Empowered by imaging biomarkers/PRIMAGE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 826494-PRIMAGE info:eu-repo/grantAgreement/ES/MCIU/FPU17-03867 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-094494-B-C21 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.7 2022 ENGINEERING, BIOMEDICAL 81 / 96 = 0.844 2022 Q4 T3 BIOPHYSICS 56 / 70 = 0.8 2022 Q4 T3 0.45 2022 Physiology (medical) 2022 Q3 Biomedical Engineering 2022 Q3 3.2 2022 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Guerrero, Pedro E. Universidad de Zaragoza (orcid)0000-0003-2612-9235 Camacho-Gomez, Daniel Universidad de Zaragoza Hervas-Raluy, Silvia Universidad de Zaragoza (orcid)0000-0001-8324-5596 García-Aznar, J.M. Universidad de Zaragoza (orcid)0000-0002-9864-7683 Gómez-Benito, María José Universidad de Zaragoza (orcid)0000-0002-1878-8997 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 144, 7 (2022), 074502 [29 pp.] J. biomech. eng. JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME 0148-0731 2985472 http://zaguan.unizar.es/record/109410/files/texto_completo.pdf Postprint 1949326 http://zaguan.unizar.es/record/109410/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:109410 articulos driver 2024-03-18-12:46:55 ARTICLE