000109445 001__ 109445
000109445 005__ 20220115145336.0
000109445 0247_ $$2doi$$a10.1021/acs.cgd.9b00969
000109445 0248_ $$2sideral$$a117007
000109445 037__ $$aART-2020-117007
000109445 041__ $$aeng
000109445 100__ $$aPolino, M.
000109445 245__ $$aEnhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques
000109445 260__ $$c2020
000109445 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109445 5203_ $$aIn this work, the influence of surface topography on protein crystallization over Nafion is investigated. Two types of Nafion-based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From an analysis of the induction time, nucleation, and crystal growth rate of trypsin from bovine pancreas, all of the patterned Nafion-based membranes show an enhanced nucleation and crystal growth. To provide additional insight into the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both the structural and chemical properties of the nucleant Nafion surface.
000109445 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000109445 590__ $$a4.076$$b2020
000109445 591__ $$aCRYSTALLOGRAPHY$$b5 / 25 = 0.2$$c2020$$dQ1$$eT1
000109445 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b67 / 178 = 0.376$$c2020$$dQ2$$eT2
000109445 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b128 / 333 = 0.384$$c2020$$dQ2$$eT2
000109445 592__ $$a0.965$$b2020
000109445 593__ $$aChemistry (miscellaneous)$$c2020$$dQ1
000109445 593__ $$aMaterials Science (miscellaneous)$$c2020$$dQ1
000109445 593__ $$aCondensed Matter Physics$$c2020$$dQ1
000109445 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000109445 700__ $$aPortugal, C.A.M.
000109445 700__ $$aLe The, H.
000109445 700__ $$aTiggelaar, R.
000109445 700__ $$aEijkel, J.
000109445 700__ $$aCrespo, J.G.
000109445 700__ $$aCoelhoso, I.M.
000109445 700__ $$0(orcid)0000-0001-9897-6527$$aPina, M.P.$$uUniversidad de Zaragoza
000109445 700__ $$0(orcid)0000-0002-4758-9380$$aMallada, R.$$uUniversidad de Zaragoza
000109445 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000109445 773__ $$g20, 4 (2020), 2174-2186$$pCryst. growth des.$$tCRYSTAL GROWTH & DESIGN$$x1528-7483
000109445 8564_ $$s1934382$$uhttps://zaguan.unizar.es/record/109445/files/texto_completo.pdf$$yPostprint
000109445 8564_ $$s1585254$$uhttps://zaguan.unizar.es/record/109445/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000109445 909CO $$ooai:zaguan.unizar.es:109445$$particulos$$pdriver
000109445 951__ $$a2022-01-15-12:40:14
000109445 980__ $$aARTICLE