109445 20220115145336.0 doi 10.1021/acs.cgd.9b00969 sideral 117007 ART-2020-117007 eng Polino, M. Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques 2020 In this work, the influence of surface topography on protein crystallization over Nafion is investigated. Two types of Nafion-based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From an analysis of the induction time, nucleation, and crystal growth rate of trypsin from bovine pancreas, all of the patterned Nafion-based membranes show an enhanced nucleation and crystal growth. To provide additional insight into the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both the structural and chemical properties of the nucleant Nafion surface. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.076 2020 CRYSTALLOGRAPHY 5 / 25 = 0.2 2020 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 67 / 178 = 0.376 2020 Q2 T2 MATERIALS SCIENCE, MULTIDISCIPLINARY 128 / 333 = 0.384 2020 Q2 T2 0.965 2020 Chemistry (miscellaneous) 2020 Q1 Materials Science (miscellaneous) 2020 Q1 Condensed Matter Physics 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Portugal, C.A.M. Le The, H. Tiggelaar, R. Eijkel, J. Crespo, J.G. Coelhoso, I.M. Pina, M.P. Universidad de Zaragoza (orcid)0000-0001-9897-6527 Mallada, R. Universidad de Zaragoza (orcid)0000-0002-4758-9380 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 20, 4 (2020), 2174-2186 Cryst. growth des. CRYSTAL GROWTH & DESIGN 1528-7483 1934382 http://zaguan.unizar.es/record/109445/files/texto_completo.pdf Postprint 1585254 http://zaguan.unizar.es/record/109445/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:109445 articulos driver 2022-01-15-12:40:14 ARTICLE