000109539 001__ 109539
000109539 005__ 20230519145558.0
000109539 0247_ $$2doi$$a10.1128/mBio.02315-21
000109539 0248_ $$2sideral$$a127180
000109539 037__ $$aART-2021-127180
000109539 041__ $$aeng
000109539 100__ $$aRuiz-Rodríguez, P
000109539 245__ $$aEvolutionary and phenotypic characterization of two spike mutations in European lineage 20E of SARS-CoV-2.
000109539 260__ $$c2021
000109539 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109539 5203_ $$aWe have detected two mutations in the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at amino acid positions 1163 and 1167 that appeared independently in multiple transmission clusters and different genetic backgrounds. Furthermore, both mutations appeared together in a cluster of 1,627 sequences belonging to clade 20E. This cluster is characterized by 12 additional single nucleotide polymorphisms but no deletions. The available structural information on the S protein in the pre- and postfusion conformations predicts that both mutations confer rigidity, which could potentially decrease viral fitness. Accordingly, we observed reduced infectivity of this spike genotype relative to the ancestral 20E sequence in vitro, and the levels of viral RNA in nasopharyngeal swabs were not significantly higher. Furthermore, the mutations did not impact thermal stability or antibody neutrali- zation by sera from vaccinated individuals but moderately reduce neutralization by convalescent-phase sera from the early stages of the pandemic. Despite multi- ple successful appearances of the two spike mutations during the first year of SARS-CoV-2 evolution, the genotype with both mutations was displaced upon the expansion of the 20I (Alpha) variant. The midterm fate of the genotype investi- gated was consistent with the lack of advantage observed in the clinical and ex- perimental data.
000109539 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109539 590__ $$a7.786$$b2021
000109539 592__ $$a2.767$$b2021
000109539 594__ $$a11.2$$b2021
000109539 591__ $$aMICROBIOLOGY$$b22 / 138 = 0.159$$c2021$$dQ1$$eT1
000109539 593__ $$aVirology$$c2021$$dQ1
000109539 593__ $$aMicrobiology$$c2021$$dQ1
000109539 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109539 700__ $$aFrancés-Gómez, C
000109539 700__ $$aChiner-Oms, A
000109539 700__ $$aLópez, MG
000109539 700__ $$aJiménez-Serrano, S
000109539 700__ $$aCancino-Muñoz, I
000109539 700__ $$aRuiz-Hueso, P
000109539 700__ $$aTorres-Puente, M
000109539 700__ $$aAlma Bracho, M
000109539 700__ $$aD’Auria, G
000109539 700__ $$aMartinez-Priego, L
000109539 700__ $$aGuerreiro, M
000109539 700__ $$aMontero-Alonso, M
000109539 700__ $$aGómez, MD
000109539 700__ $$aPiñana, JL
000109539 700__ $$aGonzález-Candelas, F
000109539 700__ $$aComas, I
000109539 700__ $$aMarina, A
000109539 700__ $$aGeller, R
000109539 700__ $$aCoscolla, M.
000109539 700__ $$0(orcid)0000-0001-5134-1006$$aBenito-Ruesca, R$$uUniversidad de Zaragoza
000109539 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000109539 773__ $$g12 (2021), e02315-21 [19 pp.]$$pMBIO$$tMBIO$$x2161-2129
000109539 8564_ $$s2899633$$uhttps://zaguan.unizar.es/record/109539/files/texto_completo.pdf$$yVersión publicada
000109539 8564_ $$s2543487$$uhttps://zaguan.unizar.es/record/109539/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109539 909CO $$ooai:zaguan.unizar.es:109539$$particulos$$pdriver
000109539 951__ $$a2023-05-18-15:58:08
000109539 980__ $$aARTICLE