109570 20230519145342.0 doi 10.1007/s00418-021-01965-2 sideral 122522 ART-2021-122522 eng Iruzubieta, Pablo Primary cilia presence and implications in bladder cancer progression and invasiveness 2021 Access copy available to the general public Unrestricted Urothelial bladder cancer is the tenth most common cancer worldwide. It is divided into muscle and non-muscle invading bladder cancer. Primary cilia have been related to several cancer hallmarks such as proliferation, epithelial-to-mesenchymal transition (EMT) or tumoral progression mainly through signaling pathways as Hedgehog (Hh). In the present study, we used immunohistochemical and ultrastructural techniques in human tissues of healthy bladder, non-muscle-invasive bladder cancer (NMIBC) and muscle-invasive bladder cancer (MIBC) to study and clarify the activation of epithelial-to-mesenchymal transition and Hedgehog signaling pathway and the presence of primary cilia. Thus, we found a clear correlation between EMT and Hedgehog activation and bladder cancer stage and progression. Moreover, we identified the presence of primary cilia in these tissues. Interestingly, we found that in NMIBC, some ciliated cells cross the basement membrane and localized in lamina propria, near blood vessels. These results show a correlation between EMT beginning from urothelial basal cells and primary cilia assembly and suggest a potential implication of this structure in tumoral migration and invasiveness (likely in a Hh-dependent way). Hence, primary cilia may play a fundamental role in urothelial bladder cancer progression and suppose a potential therapeutic target. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 2.531 2021 MICROSCOPY 4 / 9 = 0.444 2021 Q2 T2 CELL BIOLOGY 163 / 195 = 0.836 2021 Q4 T3 0.944 2021 Histology 2021 Q1 Molecular Biology 2021 Q1 Medical Laboratory Technology 2021 Q1 7.3 2021 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Castiella, Tomás (orcid)0000-0001-7453-2470 Monleón, Eva Universidad de Zaragoza (orcid)0000-0002-7453-1766 Berga, Carmen Muñoz, Guillermo Universidad de Zaragoza (orcid)0000-0001-8567-4327 Junquera, Concepción. Universidad de Zaragoza (orcid)0000-0002-9951-1075 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1013 020 Universidad de Zaragoza Dpto. Cirugía Área Anatomía Patológica 155 (2021), 547–560 Histochem. cell biol. HISTOCHEMISTRY AND CELL BIOLOGY 0948-6143 7681217 http://zaguan.unizar.es/record/109570/files/texto_completo.pdf Postprint 2417243 http://zaguan.unizar.es/record/109570/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:109570 articulos driver 2023-05-18-13:16:45 ARTICLE