000109581 001__ 109581
000109581 005__ 20230519145357.0
000109581 0247_ $$2doi$$a10.3390/polym13172958
000109581 0248_ $$2sideral$$a125738
000109581 037__ $$aART-2021-125738
000109581 041__ $$aeng
000109581 100__ $$aMartinez, A.
000109581 245__ $$aNano-second laser interference photoembossed microstructures for enhanced cell alignment
000109581 260__ $$c2021
000109581 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109581 5203_ $$aPhotoembossing is a powerful photolithographic technique to prepare surface relief structures relying on polymerization-induced diffusion in a solventless development step. Conveniently, surface patterns are formed by two or more interfering laser beams without the need for a lithographic mask. The use of nanosecond pulsed light-based interference lithography strengthens the pattern resolution through the absence of vibrational line pattern distortions. Typically, a conventional photoembossing protocol consists of an exposure step at room temperature that is followed by a thermal development step at high temperature. In this work, we explore the possibility to perform the pulsed holographic exposure directly at the development temperature. The surface relief structures generated using this modified photoembossing protocol are compared with those generated using the conventional one. Importantly, the enhancement of surface relief height has been observed by exposing the samples directly at the development temperature, reaching approximately double relief heights when compared to samples obtained using the conventional protocol. Advantageously, the light dose needed to reach the optimum height and the amount of photoinitiator can be substantially reduced in this modified protocol, demonstrating it to be a more efficient process for surface relief generation in photopolymers. Kidney epithelial cell alignment studies on substrates with relief-height optimized structures generated using the two described protocols demonstrate improved cell alignment in samples generated with exposure directly at the development temperature, highlighting the relevance of the height enhancement reached by this method. Although cell alignment is well-known to be enhanced by increasing the relief height of the polymeric grating, our work demonstrates nano-second laser interference photoembossing as a powerful tool to easily prepare polymeric gratings with tunable topography in the range of interest for fundamental cell alignment studies.
000109581 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/BIO2017-84246-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/DI2017-09585
000109581 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109581 590__ $$a4.967$$b2021
000109581 592__ $$a0.726$$b2021
000109581 594__ $$a5.7$$b2021
000109581 591__ $$aPOLYMER SCIENCE$$b16 / 90 = 0.178$$c2021$$dQ1$$eT1
000109581 593__ $$aPolymers and Plastics$$c2021$$dQ1
000109581 593__ $$aChemistry (miscellaneous)$$c2021$$dQ1
000109581 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109581 700__ $$aGonzález-Lana, S.
000109581 700__ $$0(orcid)0000-0003-0641-3407$$aAsín, L.
000109581 700__ $$0(orcid)0000-0003-1081-8482$$aMartínez de la Fuente, J.
000109581 700__ $$aBastiaansen, C. W. M.
000109581 700__ $$aBroer, D. J.
000109581 700__ $$0(orcid)0000-0003-3900-2866$$aSánchez-Somolinos, C.
000109581 773__ $$g13, 17 (2021), 2958 [15 pp.]$$pPolymers (Basel)$$tPolymers$$x2073-4360
000109581 8564_ $$s495103$$uhttps://zaguan.unizar.es/record/109581/files/texto_completo.pdf$$yVersión publicada
000109581 8564_ $$s2817937$$uhttps://zaguan.unizar.es/record/109581/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109581 909CO $$ooai:zaguan.unizar.es:109581$$particulos$$pdriver
000109581 951__ $$a2023-05-18-13:33:34
000109581 980__ $$aARTICLE