000109583 001__ 109583
000109583 005__ 20240319080946.0
000109583 0247_ $$2doi$$a10.1136/archdischild-2021-322463
000109583 0248_ $$2sideral$$a125742
000109583 037__ $$aART-2022-125742
000109583 041__ $$aeng
000109583 100__ $$aBauerl, C..
000109583 245__ $$aSARS-CoV-2 RNA and antibody detection in breast milk from a prospective multicentre study in Spain
000109583 260__ $$c2022
000109583 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109583 5203_ $$aObjectives: To develop and validate a specific protocol for SARS-CoV-2 detection in breast milk matrix and to determine the impact of maternal SARS-CoV-2 infection on the presence, concentration and persistence of specific SARS-CoV-2 antibodies. Design and patients: This is a prospective, multicentre longitudinal study (April–December 2020) in 60 mothers with SARS-CoV-2 infection and/or who have recovered from COVID-19. A control group of 13 women before the pandemic were also included. Setting: Seven health centres from different provinces in Spain. Main outcome measures: Presence of SARS-CoV-2 RNA in breast milk, targeting the N1 region of the nucleocapsid gene and the envelope (E) gene; presence and levels of SARS-CoV-2-specific immunoglobulins (Igs)—IgA, IgG and IgM—in breast milk samples from patients with COVID-19. Results: All breast milk samples showed negative results for presence of SARS-CoV-2 RNA. We observed high intraindividual and interindividual variability in the antibody response to the receptor-binding domain of the SARS-CoV-2 spike protein for each of the three isotypes IgA, IgM and IgG. Main Protease (MPro) domain antibodies were also detected in milk. 82.9% (58 of 70) of milk samples were positive for at least one of the three antibody isotypes, with 52.9% of these positive for all three Igs. Positivity rate for IgA was relatively stable over time (65.2%–87.5%), whereas it raised continuously for IgG (from 47.8% for the first 10 days to 87.5% from day 41 up to day 206 post-PCR confirmation). Conclusions: Our study confirms the safety of breast feeding and highlights the relevance of virus-specific SARS-CoV-2 antibody transfer. This study provides crucial data to support official breastfeeding recommendations based on scientific evidence.
000109583 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000109583 590__ $$a4.4$$b2022
000109583 591__ $$aPEDIATRICS$$b13 / 130 = 0.1$$c2022$$dQ1$$eT1
000109583 594__ $$a9.8$$b2022
000109583 592__ $$a1.861$$b2022
000109583 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000109583 593__ $$aPediatrics, Perinatology and Child Health$$c2022$$dQ1
000109583 593__ $$aObstetrics and Gynecology$$c2022$$dQ1
000109583 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109583 700__ $$aRandazzo, W.
000109583 700__ $$aSánchez, G.
000109583 700__ $$aSelma-Royo, M.
000109583 700__ $$aGarcía Verdevio, E.
000109583 700__ $$aMartínez, L.
000109583 700__ $$aParra-Llorca, A.
000109583 700__ $$aLerín, C.
000109583 700__ $$aFumadó, V.
000109583 700__ $$aCrovetto, F.
000109583 700__ $$aCrispi, F.
000109583 700__ $$aPérez-Cano, F. J.
000109583 700__ $$0(orcid)0000-0002-7985-9912$$aRodríguez, G.$$uUniversidad de Zaragoza
000109583 700__ $$aRuiz-Redondo, G.
000109583 700__ $$aCampoy, C.
000109583 700__ $$aMartínez-Costa, C.
000109583 700__ $$aCollado, M. C. on behalf of MilkCORONA study team
000109583 7102_ $$11011$$2670$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Pediatría
000109583 773__ $$g107, 2 (2022), 217-221$$pArch. dis. child., Fetal neonatal ed.$$tArchives of Disease in Childhood-Fetal and Neonatal Edition$$x1359-2998
000109583 8564_ $$s6432440$$uhttps://zaguan.unizar.es/record/109583/files/texto_completo.pdf$$yVersión publicada
000109583 8564_ $$s3186944$$uhttps://zaguan.unizar.es/record/109583/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109583 909CO $$ooai:zaguan.unizar.es:109583$$particulos$$pdriver
000109583 951__ $$a2024-03-18-12:34:42
000109583 980__ $$aARTICLE