109583 20240319080946.0 doi 10.1136/archdischild-2021-322463 sideral 125742 ART-2022-125742 eng Bauerl, C.. SARS-CoV-2 RNA and antibody detection in breast milk from a prospective multicentre study in Spain 2022 Access copy available to the general public Unrestricted Objectives: To develop and validate a specific protocol for SARS-CoV-2 detection in breast milk matrix and to determine the impact of maternal SARS-CoV-2 infection on the presence, concentration and persistence of specific SARS-CoV-2 antibodies. Design and patients: This is a prospective, multicentre longitudinal study (April–December 2020) in 60 mothers with SARS-CoV-2 infection and/or who have recovered from COVID-19. A control group of 13 women before the pandemic were also included. Setting: Seven health centres from different provinces in Spain. Main outcome measures: Presence of SARS-CoV-2 RNA in breast milk, targeting the N1 region of the nucleocapsid gene and the envelope (E) gene; presence and levels of SARS-CoV-2-specific immunoglobulins (Igs)—IgA, IgG and IgM—in breast milk samples from patients with COVID-19. Results: All breast milk samples showed negative results for presence of SARS-CoV-2 RNA. We observed high intraindividual and interindividual variability in the antibody response to the receptor-binding domain of the SARS-CoV-2 spike protein for each of the three isotypes IgA, IgM and IgG. Main Protease (MPro) domain antibodies were also detected in milk. 82.9% (58 of 70) of milk samples were positive for at least one of the three antibody isotypes, with 52.9% of these positive for all three Igs. Positivity rate for IgA was relatively stable over time (65.2%–87.5%), whereas it raised continuously for IgG (from 47.8% for the first 10 days to 87.5% from day 41 up to day 206 post-PCR confirmation). Conclusions: Our study confirms the safety of breast feeding and highlights the relevance of virus-specific SARS-CoV-2 antibody transfer. This study provides crucial data to support official breastfeeding recommendations based on scientific evidence. info:eu-repo/semantics/openAccess by-nc http://creativecommons.org/licenses/by-nc/3.0/es/ 4.4 2022 PEDIATRICS 13 / 130 = 0.1 2022 Q1 T1 1.861 2022 Medicine (miscellaneous) 2022 Q1 Pediatrics, Perinatology and Child Health 2022 Q1 Obstetrics and Gynecology 2022 Q1 9.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Randazzo, W. Sánchez, G. Selma-Royo, M. García Verdevio, E. Martínez, L. Parra-Llorca, A. Lerín, C. Fumadó, V. Crovetto, F. Crispi, F. Pérez-Cano, F. J. Rodríguez, G. Universidad de Zaragoza (orcid)0000-0002-7985-9912 Ruiz-Redondo, G. Campoy, C. Martínez-Costa, C. Collado, M. C. on behalf of MilkCORONA study team 1011 670 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Pediatría 107, 2 (2022), 217-221 Arch. dis. child., Fetal neonatal ed. Archives of Disease in Childhood-Fetal and Neonatal Edition 1359-2998 6432440 http://zaguan.unizar.es/record/109583/files/texto_completo.pdf Versión publicada 3186944 http://zaguan.unizar.es/record/109583/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:109583 articulos driver 2024-03-18-12:34:42 ARTICLE