000109620 001__ 109620
000109620 005__ 20230519145432.0
000109620 0247_ $$2doi$$a10.1039/d1fo03178c
000109620 0248_ $$2sideral$$a125712
000109620 037__ $$aART-2021-125712
000109620 041__ $$aeng
000109620 100__ $$0(orcid)0000-0001-6969-1055$$aLópez, Víctor
000109620 245__ $$aNeuroprotective and anxiolytic potential of green rooibos (Aspalathus linearis) polyphenolic extract
000109620 260__ $$c2021
000109620 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109620 5203_ $$aSouth African rooibos (Aspalathus linearis) tea is globally consumed for its health benefits and caffeine free nature, but no information is available on the neuroprotective capacity of (unfermented) green rooibos. Our aim was to investigate the cytoprotective activity of green rooibos in neuronal cells, including probing antioxidant and enzyme inhibitory properties that could explain observed effects in these cells. We also investigated the anxiolytic potential of green rooibos using zebrafish larval models. Green rooibos extract (Green oxithin (TM)) was assessed for its neuroprotective potential in Neuro-2a cells treated with different concentrations of the extract (12.5-25-50-100 mu g mL(-1)) and different concentrations of hydrogen peroxide (250 or 125 mu M) as oxidizing agent. Cell viability (MTT) and redox status (intracellular ROS) were also quantified in these cells. Antioxidant properties of the extract were quantified using cell-free systems (DPPH, ORAC and xanthine/xanthine oxidase), and potential neuroprotection evaluated in terms of its potential to inhibit key enzymes of the CNS (monoamine oxidase A (MOA-A), acetylcholinesterase (AChE) and tyrosinase (TYR)). Results demonstrated that green rooibos extract exerted significant cytoprotective properties in Neuro-2a cells, particularly when exposed to lethal 250 mu M hydrogen peroxide, increasing cell survival by more than 100%. This may be ascribed (at least partially) to its capacity to limit intracellular ROS accumulation in these cells. Data from cell-free systems confirmed that green rooibos was able to scavenge free radicals (synthetic and physiological) in a dose dependent manner with a similar profile activity to vitamins C and E. Green rooibos also acted as a moderate MAO-A inhibitor, but had no significant effect on AChE or TYR. Finally, zebrafish larvae treated with lower doses of green rooibos demonstrated a significant anxiolytic effect in the light-dark anxiety model. Using the PTZ excitotoxicity model, green rooibos was shown to rescue GABA receptor signalling, which together with its demonstrated inhibition of MAO-A, may account for the anxiolytic outcome. Current data confirms that green rooibos could be considered a functional brain food and may be a good option as starting ingredient in the development of new nutraceuticals.
000109620 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000109620 590__ $$a6.317$$b2021
000109620 592__ $$a1.011$$b2021
000109620 594__ $$a7.8$$b2021
000109620 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b24 / 144 = 0.167$$c2021$$dQ1$$eT1
000109620 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1
000109620 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 297 = 0.226$$c2021$$dQ1$$eT1
000109620 593__ $$aFood Science$$c2021$$dQ1
000109620 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109620 700__ $$aCásedas, Guillermo
000109620 700__ $$aPetersen-Ross, Kelly
000109620 700__ $$aPowrie, Yigael
000109620 700__ $$aSmith, Carine
000109620 773__ $$g13, 1 (2021), 91-101$$pFOOD & FUNCTION$$tFOOD & FUNCTION$$x2042-6496
000109620 8564_ $$s2682250$$uhttps://zaguan.unizar.es/record/109620/files/texto_completo.pdf$$yVersión publicada
000109620 8564_ $$s2679425$$uhttps://zaguan.unizar.es/record/109620/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109620 909CO $$ooai:zaguan.unizar.es:109620$$particulos$$pdriver
000109620 951__ $$a2023-05-18-14:19:47
000109620 980__ $$aARTICLE