000109665 001__ 109665
000109665 005__ 20230519145526.0
000109665 0247_ $$2doi$$a10.3390/membranes11080549
000109665 0248_ $$2sideral$$a125407
000109665 037__ $$aART-2021-125407
000109665 041__ $$aeng
000109665 100__ $$aPolino, M.
000109665 245__ $$aProtein Crystallization in a Microfluidic Contactor with Nafion®117 Membranes
000109665 260__ $$c2021
000109665 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109665 5203_ $$aProtein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal’s derivatization conditions. Herein, this demanding step was addressed by the development of a high-throughput and low-budget microfluidic platform consisting of an ion exchange membrane (117 Nafion® membrane) sandwiched between a channel layer (stripping phase compartment) and a wells layer (feed phase compartment) forming 75 independent micro-contactors. This microfluidic device allows for a simultaneous and independent screening of multiple protein crystallization and crystal derivatization conditions, using Hen Egg White Lysozyme (HEWL) as the model protein and Hg2+ as the derivatizing agent. This microdevice offers well-regulated crystallization and subsequent crystal derivatization processes based on the controlled transport of water and ions provided by the 117 Nafion® membrane. Diffusion coefficients of water and the derivatizing agent (Hg2+) were evaluated, showing the positive influence of the protein drop volume on the number of crystals and crystal size. This microfluidic system allowed for crystals with good structural stability and high X-ray diffraction quality and, thus, it is regarded as an efficient tool that may contribute to the enhancement of the proteins’ crystals structural resolution.
000109665 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109665 590__ $$a4.562$$b2021
000109665 592__ $$a0.517$$b2021
000109665 594__ $$a3.7$$b2021
000109665 591__ $$aPOLYMER SCIENCE$$b21 / 90 = 0.233$$c2021$$dQ1$$eT1
000109665 593__ $$aProcess Chemistry and Technology$$c2021$$dQ2
000109665 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b132 / 345 = 0.383$$c2021$$dQ2$$eT2
000109665 593__ $$aChemical Engineering (miscellaneous)$$c2021$$dQ2
000109665 591__ $$aCHEMISTRY, PHYSICAL$$b69 / 165 = 0.418$$c2021$$dQ2$$eT2
000109665 591__ $$aENGINEERING, CHEMICAL$$b47 / 143 = 0.329$$c2021$$dQ2$$eT1
000109665 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109665 700__ $$aRho, H. S.
000109665 700__ $$0(orcid)0000-0001-9897-6527$$aPina, M. P.$$uUniversidad de Zaragoza
000109665 700__ $$0(orcid)0000-0002-4758-9380$$aMallada, R.$$uUniversidad de Zaragoza
000109665 700__ $$aCarvalho, A. L.
000109665 700__ $$aRomão, M. J.
000109665 700__ $$aCoelhoso, Isabel
000109665 700__ $$aGardeniers, J. G. E.
000109665 700__ $$aCrespo, J. G.
000109665 700__ $$aPortugal, Carla A. M.
000109665 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000109665 773__ $$g11, 8 (2021), 549 [18 pp.]$$pMembranes$$tMembranes$$x2077-0375
000109665 8564_ $$s4729447$$uhttps://zaguan.unizar.es/record/109665/files/texto_completo.pdf$$yVersión publicada
000109665 8564_ $$s2677965$$uhttps://zaguan.unizar.es/record/109665/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109665 909CO $$ooai:zaguan.unizar.es:109665$$particulos$$pdriver
000109665 951__ $$a2023-05-18-15:26:35
000109665 980__ $$aARTICLE