109665 20230519145526.0 doi 10.3390/membranes11080549 sideral 125407 ART-2021-125407 eng Polino, M. Protein Crystallization in a Microfluidic Contactor with Nafion®117 Membranes 2021 Access copy available to the general public Unrestricted Protein crystallization still remains mostly an empirical science, as the production of crystals with the required quality for X-ray analysis is dependent on the intensive screening of the best protein crystallization and crystal’s derivatization conditions. Herein, this demanding step was addressed by the development of a high-throughput and low-budget microfluidic platform consisting of an ion exchange membrane (117 Nafion® membrane) sandwiched between a channel layer (stripping phase compartment) and a wells layer (feed phase compartment) forming 75 independent micro-contactors. This microfluidic device allows for a simultaneous and independent screening of multiple protein crystallization and crystal derivatization conditions, using Hen Egg White Lysozyme (HEWL) as the model protein and Hg2+ as the derivatizing agent. This microdevice offers well-regulated crystallization and subsequent crystal derivatization processes based on the controlled transport of water and ions provided by the 117 Nafion® membrane. Diffusion coefficients of water and the derivatizing agent (Hg2+) were evaluated, showing the positive influence of the protein drop volume on the number of crystals and crystal size. This microfluidic system allowed for crystals with good structural stability and high X-ray diffraction quality and, thus, it is regarded as an efficient tool that may contribute to the enhancement of the proteins’ crystals structural resolution. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.562 2021 POLYMER SCIENCE 21 / 90 = 0.233 2021 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 132 / 345 = 0.383 2021 Q2 T2 CHEMISTRY, PHYSICAL 69 / 165 = 0.418 2021 Q2 T2 ENGINEERING, CHEMICAL 47 / 143 = 0.329 2021 Q2 T1 0.517 2021 Process Chemistry and Technology 2021 Q2 Chemical Engineering (miscellaneous) 2021 Q2 3.7 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Rho, H. S. Pina, M. P. Universidad de Zaragoza (orcid)0000-0001-9897-6527 Mallada, R. Universidad de Zaragoza (orcid)0000-0002-4758-9380 Carvalho, A. L. Romão, M. J. Coelhoso, Isabel Gardeniers, J. G. E. Crespo, J. G. Portugal, Carla A. M. 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 11, 8 (2021), 549 [18 pp.] Membranes Membranes 2077-0375 4729447 http://zaguan.unizar.es/record/109665/files/texto_completo.pdf Versión publicada 2677965 http://zaguan.unizar.es/record/109665/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:109665 articulos driver 2023-05-18-15:26:35 ARTICLE