000109681 001__ 109681
000109681 005__ 20230519145529.0
000109681 0247_ $$2doi$$a10.20870/oeno-one.2021.55.3.4688
000109681 0248_ $$2sideral$$a126984
000109681 037__ $$aART-2021-126984
000109681 041__ $$aeng
000109681 100__ $$aCalvo, H
000109681 245__ $$aComparison of efficacy and modes of action of two high-potential biocontrol Bacillus strains and commercial biocontrol products against Botrytis cinerea in table grapes
000109681 260__ $$c2021
000109681 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109681 5203_ $$aIn table grapes (cv. Thomson Seedless), the antifungal activity against Botrytis cinerea was further analysed and compared between two high potential bacterial biological control agent (BCA) strains, Bacillus velezensis BUZ-14 and B. ginsengihumi S38. Two commercial biocontrol products (BPs), served as standards of comparison, Amylo-X (R) and Serenade Max (R), also based on Bacillus BCA strains. The main mode of action quantified for all the strains was antibiosis due to hydrosoluble and volatile metabolites and their combinations. The BUZ-14 strain was the most active BCA strain, demonstrating significant disease reduction exceeding 60 % when used in the culture form grown in 863 medium, including living cells (LCs) and cell-free supernatant (CFS). Both BPs exhibited significantly reduced efficacy of their CFS fraction (< 10 %) compared with that of the two BCA strains, confirming their high antibiosis potential. The novel methodology allowed us to demonstrate the significant effect of the BCA culture medium on volatilome (VOC) antagonist efficacy. The S38 strain achieved the highest disease reduction (90 %) owing to the greatest production of VOCs in the richest MOLP (Medium Optimum Lipopetide Production) culture medium, whereas grape juice was the least favourable medium for VOC efficacy for both bacterial strains (BUZ14 and S38). The overall poor activity of living Bacillus cells in all the BCA and BPs tested is discussed based on the low capacity of the BCA strains to grow in the berry. Then, the presence of living cells is also discussed with the possibility that these cells are not required in field applications of such BCA strains in this genus. Moreover, different environmental suboptimal conditions, including temperature (22 and 27 degrees C) and relative humidity (RH) (100 and 85-95 %), were tested, and BUZ-14 exhibited the highest Botrytis reduction at both temperatures and RH values. However, no significant differences were observed between temperatures or RH values for the same BCA. Further studies in vineyard conditions and applications, such as biofumigation or active packaging, will be performed to confirm the new findings reported in this investigation.
000109681 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109681 590__ $$a3.0$$b2021
000109681 592__ $$a0.491$$b2021
000109681 594__ $$a3.3$$b2021
000109681 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b81 / 144 = 0.562$$c2021$$dQ3$$eT2
000109681 593__ $$aHorticulture$$c2021$$dQ1
000109681 593__ $$aFood Science$$c2021$$dQ1
000109681 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109681 700__ $$aRoudet, J
000109681 700__ $$aGracia, AP
000109681 700__ $$0(orcid)0000-0001-6316-385X$$aVenturini Crespo, M. E$$uUniversidad de Zaragoza
000109681 700__ $$aFermaud, M
000109681 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000109681 773__ $$g55, 3 (2021), 229-243$$pOENO One$$tOeno One$$x2494-1271
000109681 8564_ $$s658146$$uhttps://zaguan.unizar.es/record/109681/files/texto_completo.pdf$$yVersión publicada
000109681 8564_ $$s2184185$$uhttps://zaguan.unizar.es/record/109681/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109681 909CO $$ooai:zaguan.unizar.es:109681$$particulos$$pdriver
000109681 951__ $$a2023-05-18-15:29:23
000109681 980__ $$aARTICLE