000109685 001__ 109685
000109685 005__ 20230519145544.0
000109685 0247_ $$2doi$$a10.3390/plants10122664
000109685 0248_ $$2sideral$$a126509
000109685 037__ $$aART-2021-126509
000109685 041__ $$aeng
000109685 100__ $$aDecena M.A.$$uUniversidad de Zaragoza
000109685 245__ $$aComparative genomics, evolution, and drought-induced expression of dehydrin genes in model Brachypodium grasses
000109685 260__ $$c2021
000109685 5060_ $$aAccess copy available to the general public$$fUnrestricted
000109685 5203_ $$aDehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachy-podium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
000109685 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-108195GB-I00
000109685 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000109685 590__ $$a4.658$$b2021
000109685 592__ $$a0.765$$b2021
000109685 594__ $$a3.6$$b2021
000109685 591__ $$aPLANT SCIENCES$$b39 / 240 = 0.162$$c2021$$dQ1$$eT1
000109685 593__ $$aPlant Science$$c2021$$dQ1
000109685 593__ $$aEcology, Evolution, Behavior and Systematics$$c2021$$dQ1
000109685 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000109685 700__ $$aGálvez-Rojas S.
000109685 700__ $$aAgostini F.
000109685 700__ $$0(orcid)0000-0002-4845-4242$$aSancho R.$$uUniversidad de Zaragoza
000109685 700__ $$aContreras-Moreira B.
000109685 700__ $$aDes Marais D.L.
000109685 700__ $$aHernandez P.
000109685 700__ $$0(orcid)0000-0001-7793-5259$$aCatalán P.$$uUniversidad de Zaragoza
000109685 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000109685 773__ $$g10, 12 (2021), 2664 [29 pp]$$tPlants$$x2223-7747
000109685 8564_ $$s8264407$$uhttps://zaguan.unizar.es/record/109685/files/texto_completo.pdf$$yVersión publicada
000109685 8564_ $$s2814573$$uhttps://zaguan.unizar.es/record/109685/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000109685 909CO $$ooai:zaguan.unizar.es:109685$$particulos$$pdriver
000109685 951__ $$a2023-05-18-15:42:17
000109685 980__ $$aARTICLE