000110580 001__ 110580 000110580 005__ 20230519145610.0 000110580 0247_ $$2doi$$a10.3389/fmed.2021.673408 000110580 0248_ $$2sideral$$a127278 000110580 037__ $$aART-2021-127278 000110580 041__ $$aeng 000110580 100__ $$0(orcid)0000-0002-0266-1588$$aPérez Piñero, M. 000110580 245__ $$aComparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both 000110580 260__ $$c2021 000110580 5060_ $$aAccess copy available to the general public$$fUnrestricted 000110580 5203_ $$aBackground: Antibiotic resistance and impaired wound healing are major concerns in S. aureus superficial skin infections, and new therapies are needed. Antimicrobial photodynamic therapy (aPDT) is a new therapeutic approach for infections, but it also improves healing in many wound models. Objective: To compare the antimicrobial activity and the effects on wound healing of aPDT based on Methylene Blue (MB-aPDT) with mupirocin treatment, either alone or in combination, in superficial skin wounds of S. aureus-infected mice. Additionally, to evaluate the clinical, microbiological, and cosmetic effects on wound healing. Materials and Methods: A superficial skin infection model of S. aureus was established in SKH-1 mice. Infected wounds were treated with MB-aPDT, MB-aPDT with a daily topical mupirocin or only with mupirocin. No treatment was carried out in control animals. Daily clinical and microbiological examinations were performed until complete clinical wound healing. Histopathological studies and statistical analysis were performed at the end of the study. Results: MB-aPDT treatment induced the best wound healing compared to mupirocin alone or to mupirocin plus MB-aPDT. Superficial contraction at 24 h and a greater reduction in size at 48 h, quicker detachment of the crust, less scaling, and absence of scars were observed. Histopathological studies correlated with clinical and gross findings. By contrast, mupirocin showed the highest logaritmic reduction of S. aureus. Conclusions: MB-aPDT and mupirocin treatments are effective in a murine superficial skin infection model of S. aureus. One session of MB-aPDT was the best option for clinical wound healing and cosmetic results. The addition of mupirocin to MB-aPDT treatment improved antimicrobial activity; however, it did not enhance wound healing. No synergistic antibacterial effects were detected. © Copyright © 2021 Pérez, Robres, Moreno, Bolea, Verde, Pérez-Laguna, Aspiroz, Gilaberte and Rezusta. 000110580 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000110580 590__ $$a5.058$$b2021 000110580 592__ $$a1.179$$b2021 000110580 594__ $$a3.4$$b2021 000110580 591__ $$aMEDICINE, GENERAL & INTERNAL$$b53 / 172 = 0.308$$c2021$$dQ2$$eT1 000110580 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1 000110580 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000110580 700__ $$aRobres P. 000110580 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno Burgos, B.$$uUniversidad de Zaragoza 000110580 700__ $$0(orcid)0000-0002-2746-3932$$aBolea Bailo, R.$$uUniversidad de Zaragoza 000110580 700__ $$0(orcid)0000-0003-2957-1379$$aVerde Arribas M.T.$$uUniversidad de Zaragoza 000110580 700__ $$aPérez-Laguna V. 000110580 700__ $$aAspiroz C. 000110580 700__ $$aGilaberte Y. 000110580 700__ $$aRezusta A. 000110580 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000110580 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000110580 773__ $$g8 (2021), 673408$$pFront. med.$$tFrontiers in Medicine$$x2296-858X 000110580 8564_ $$s1796568$$uhttps://zaguan.unizar.es/record/110580/files/texto_completo.pdf$$yVersión publicada 000110580 8564_ $$s2296565$$uhttps://zaguan.unizar.es/record/110580/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000110580 909CO $$ooai:zaguan.unizar.es:110580$$particulos$$pdriver 000110580 951__ $$a2023-05-18-16:07:50 000110580 980__ $$aARTICLE