110627 20240319081002.0 doi 10.3389/fvets.2021.805004 sideral 127501 ART-2022-127501 eng Badiola, J. J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 SARS-CoV-2 outbreak on a Spanish mink farm: epidemiological, molecular, and pathological studies 2022 Access copy available to the general public Unrestricted Farmed minks have been reported to be highly susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and may represent a risk to humans. In this study, we describe the first outbreak of SARS-CoV-2 occurred on a mink farm in Spain, between June and July 2020, involving 92,700 animals. The outbreak started shortly after some farm workers became seropositive for SARS-CoV-2. Minks showed no clinical signs compatible with SARS-CoV-2 infection throughout the outbreak. Samples from 98 minks were collected for histopathological, serological, and molecular studies. Twenty out of 98 (20.4%) minks were positive by RT-qPCR and 82 out 92 (89%) seroconverted. This finding may reflect a rapid spread of the virus at the farm with most of the animals overcoming the infection. Additionally, SARS-CoV-2 was detected by RT-qPCR in 30% of brain samples from positive minks. Sequencing analysis showed that the mink sequences were not closely related with the other mink SARS-CoV-2 sequences available, and that this mink outbreak has its probable origin in one of the genetic variants that were prevalent in Spain during the first COVID-19 epidemic wave. Histological studies revealed bronchointerstitial pneumonia in some animals. Immunostaining of viral nucleocapsid was also observed in nasal turbinate tissue. Farmed minks could therefore constitute an important SARS-CoV-2 reservoir, contributing to virus spread among minks and humans. Consequently, continuous surveillance of mink farms is needed. info:eu-repo/grantAgreement/ES/ISCIII/COV20-00140 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.2 2022 VETERINARY SCIENCES 12 / 144 = 0.083 2022 Q1 T1 0.737 2022 Veterinary (miscellaneous) 2022 Q1 3.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Otero, A. Universidad de Zaragoza (orcid)0000-0001-9075-2764 Sevilla, E. Universidad de Zaragoza (orcid)0000-0002-4213-2904 Marín, B. Universidad de Zaragoza (orcid)0000-0002-1590-3347 García Martínez, M. Universidad de Zaragoza (orcid)0000-0002-8752-5146 Betancor, M. Universidad de Zaragoza (orcid)0000-0002-0388-9429 Sola, D. Universidad de Zaragoza Pérez Lázaro, S. Universidad de Zaragoza (orcid)0000-0002-2079-0711 Lozada, J. Velez, C. Chiner-Oms, Á. Comas, I. Cancino-Muñoz, I. Monleón, E Universidad de Zaragoza (orcid)0000-0002-7453-1766 Monzón, M. Universidad de Zaragoza (orcid)0000-0002-2787-9671 Acín, C. Universidad de Zaragoza (orcid)0000-0001-5105-6133 Bolea, R. Universidad de Zaragoza (orcid)0000-0002-2746-3932 Moreno, B. Universidad de Zaragoza (orcid)0000-0001-6629-6842 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 8 (2022), 805004[9 pp.] Front. vet. sci. Frontiers in Veterinary Science 2297-1769 1337713 http://zaguan.unizar.es/record/110627/files/texto_completo.pdf Versión publicada 2426718 http://zaguan.unizar.es/record/110627/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:110627 articulos driver 2024-03-18-14:14:51 ARTICLE