000110668 001__ 110668
000110668 005__ 20230914083328.0
000110668 0247_ $$2doi$$a10.3390/agronomy12020461
000110668 0248_ $$2sideral$$a127653
000110668 037__ $$aART-2022-127653
000110668 041__ $$aeng
000110668 100__ $$aSánchez Hernández, Eva
000110668 245__ $$aLignin–chitosan nanocarriers for the delivery of bioactive natural products against wood-decay phytopathogens
000110668 260__ $$c2022
000110668 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110668 5203_ $$aThe use of nanocarriers (NCs), i.e., nanomaterials capable of encapsulating drugs and releasing them selectively, is an emerging field in agriculture. In this study, the synthesis, characterization, and in vitro and in vivo testing of biodegradable NCs loaded with natural bioactive products was investigated for the control of certain phytopathogens responsible for wood degradation. In particular, NCs based on methacrylated lignin and chitosan oligomers, loaded with extracts from Rubia tinctorum, Silybum marianum, Equisetum arvense, and Urtica dioica, were first assayed in vitro against Neofusicoccum parvum, an aggressive fungus that causes cankers and diebacks in numerous woody hosts around the world. The in vitro antimicrobial activity of the most effective treatment was further explored against another fungal pathogen and two bacteria related to trunk diseases: Diplodia seriata, Xylophilus ampelinus, and Pseudomonas syringae pv. syringae, respectively. Subsequently, it was evaluated in field conditions, in which it was applied by endotherapy for the control of grapevine trunk diseases. In the in vitro mycelial growth inhibition tests, the NCs loaded with R. tinctorum resulted in EC90 concentrations of 65.8 and 91.0 μg·mL−1 against N. parvum and D. seriata, respectively. Concerning their antibacterial activity, a minimum inhibitory concentration of 37.5 μg·mL−1 was obtained for this treatment against both phytopathogens. Upon application via endotherapy on 20-year-old grapevines with clear esca and Botryosphaeria decay symptoms, no phytotoxicity effects were observed (according to SPAD and chlorophyll fluorescence measurements) and the sugar content of the grape juice was not affected either. Nonetheless, the treatment led to a noticeable decrease in foliar symptoms as well as a higher yield in the treated arms as compared to the control arms (3177 vs. 1932 g/arm), suggestive of high efficacy. Given the advantages in terms of controlled release and antimicrobial product savings, these biodegradable NCs loaded with natural extracts may deserve further research in large-scale field tests.
000110668 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000110668 590__ $$a3.7$$b2022
000110668 592__ $$a0.663$$b2022
000110668 591__ $$aAGRONOMY$$b16 / 88 = 0.182$$c2022$$dQ1$$eT1
000110668 593__ $$aAgronomy and Crop Science$$c2022$$dQ1
000110668 591__ $$aPLANT SCIENCES$$b60 / 238 = 0.252$$c2022$$dQ2$$eT1
000110668 594__ $$a5.2$$b2022
000110668 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000110668 700__ $$aLanga Lomba, Natalia
000110668 700__ $$aGonzález García, Vicente
000110668 700__ $$0(orcid)0000-0003-3009-0935$$aCasanova Gascón, José$$uUniversidad de Zaragoza
000110668 700__ $$aMartín Gil, Jesús
000110668 700__ $$aSantiago Aliste, Alberto
000110668 700__ $$aTorres Sánchez, Sergio
000110668 700__ $$0(orcid)0000-0003-2713-2786$$aMartín Ramos, Pablo$$uUniversidad de Zaragoza
000110668 7102_ $$15011$$2500$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cArea Ingeniería Agroforestal
000110668 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000110668 773__ $$g12, 2 (2022), 461 [16 pp.]$$pAgronomy (Basel)$$tAgronomy (Basel)$$x2073-4395
000110668 8564_ $$s3194052$$uhttps://zaguan.unizar.es/record/110668/files/texto_completo.pdf$$yVersión publicada
000110668 8564_ $$s2819559$$uhttps://zaguan.unizar.es/record/110668/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000110668 909CO $$ooai:zaguan.unizar.es:110668$$particulos$$pdriver
000110668 951__ $$a2023-09-13-11:11:51
000110668 980__ $$aARTICLE