110689 20250127144950.0 doi 10.1038/s41598-021-89511-x sideral 126805 ART-2021-126805 eng Comín, J. Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6 2021 Access copy available to the general public Unrestricted The purpose of this study was to increase our knowledge about Mycobacterium africanum and report the incidence and characteristics of tuberculosis (TB) due to their lineages in Aragon, Spain, over the period 2003–2019. The study includes all the cases in our region, where all the M. tuberculosis complex isolates are systematically characterised. We detected 31 cases of M. africanum among 2598 cases of TB in the period studied. TB caused by M. africanum is rare (1.19%) in our population, and it affects mainly men of economically productive age coming from West African countries. Among the isolates, Lineage (L) 6 was more frequent than L5. The genotyping of these strains identified five clusters and 13 strains with a unique pattern. The isolates’ characterisation identified a copy of IS6110 within the moaX gene, which turned out to be specific for L6. It will allow the differentiation of this lineage from the rest of MTBC with a simple PCR reaction. It remains to be established whether this polymorphism may limit M. africanum transmission. Furthermore, a mutation in the mutT2 promoter was found as specific for L6 strains, which could be related to the high variability found for L6 compared to L5. © 2021, The Author(s). info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón info:eu-repo/grantAgreement/ES/ISCIII/FIS18-0336 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.997 2021 MULTIDISCIPLINARY SCIENCES 19 / 74 = 0.257 2021 Q2 T1 1.005 2021 Multidisciplinary 2021 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Monforte, M.L. Samper, S. Iglesias, M.J. Universidad de Zaragoza (orcid)0000-0003-2266-8602 Ibarz, D. Universidad de Zaragoza Viñuelas, J. Torres, L. Sahagún, J. Lafoz, M.C. Malo, M.C. Otal, I. Universidad de Zaragoza (orcid)0000-0003-1027-7583 Aragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA) 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 1011 615 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Medic.Prevent.Salud Públ. 11, 1 (2021), 10359 [10 pp] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 1441302 http://zaguan.unizar.es/record/110689/files/texto_completo.pdf Versión publicada 2584675 http://zaguan.unizar.es/record/110689/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:110689 articulos driver 2025-01-27-14:45:30 ARTICLE