000110696 001__ 110696
000110696 005__ 20230519145604.0
000110696 0247_ $$2doi$$a10.3390/biology10111135
000110696 0248_ $$2sideral$$a127220
000110696 037__ $$aART-2021-127220
000110696 041__ $$aeng
000110696 100__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.L.$$uUniversidad de Zaragoza
000110696 245__ $$aAbility of the isas3fun method to detect sperm acrosome integrity and its potential to discriminate between high and low field fertility bulls
000110696 260__ $$c2021
000110696 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110696 5203_ $$aThe objective of the present study was to investigate whether fertility differences in bulls are reflected in variations of sperm quality when analysing only one ejaculate per male. Two experiments were performed. In the first experiment, frozen semen samples from 20 adult bulls were tested; 10 bulls had high field fertility and 10 bulls had low field fertility. Analyses of sperm motility, membrane integrity, and membrane–acrosome integrity with the ISAS3Fun method were performed. Sperm morphometry of the fluorescence sperm subpopulations obtained with the ISAS3Fun method was also analysed. Significant differences between high-and low-fertility groups were only found with the ISAS3Fun technique, specifically in sperm acrosome integrity, the proportion of spermatozoa with an intact acrosome and damaged membrane, and in sperm head width of spermatozoa with intact structures. Discriminant analyses allowed us to correctly classify 90% of sperm samples in their fertility group using sperm quality parameters. Given that only the results obtained with the ISAS3Fun technique were related to bull fertility, we performed a second experiment aimed to validate the efficacy of this technique to detect the acrosomal integrity of bull spermatozoa, comparing them with the conventional FITC-PNA/propidium iodide (PNA/PI) combination under capacitating conditions. The results indicated that the ISAS3Fun combination provided an accurate assessment of both viability and acrosomal integrity for ejaculated spermatozoa, while the PNA/PI combination underestimated the extension of acrosomal damage due to false negatives. It was concluded that the simultaneous assessment of sperm plasma membranes and acrosome integrity with the ISAS3Fun method is precise and seems to have a greater potential to discriminate between high-and low-fertility bulls than more conventional in vitro sperm quality tests. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
000110696 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/A07-17R$$9info:eu-repo/grantAgreement/EC/ETC/EFA144-16/EU/DietaPYR2$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-85030-R
000110696 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000110696 590__ $$a5.168$$b2021
000110696 592__ $$a0.903$$b2021
000110696 594__ $$a2.8$$b2021
000110696 591__ $$aBIOLOGY$$b21 / 94 = 0.223$$c2021$$dQ1$$eT1
000110696 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2021$$dQ1
000110696 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2021$$dQ1
000110696 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000110696 700__ $$0(orcid)0000-0003-1228-535X$$aPalacín, I.$$uUniversidad de Zaragoza
000110696 700__ $$aSilvestre, M.A.
000110696 700__ $$aHidalgo, C.O.
000110696 700__ $$aTamargo, C.
000110696 700__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza
000110696 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000110696 773__ $$g10, 11 (2021), 1135 [10 pp]$$tBiology$$x2079-7737
000110696 8564_ $$s889710$$uhttps://zaguan.unizar.es/record/110696/files/texto_completo.pdf$$yVersión publicada
000110696 8564_ $$s2748519$$uhttps://zaguan.unizar.es/record/110696/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000110696 909CO $$ooai:zaguan.unizar.es:110696$$particulos$$pdriver
000110696 951__ $$a2023-05-18-16:02:40
000110696 980__ $$aARTICLE