000110758 001__ 110758
000110758 005__ 20220223124038.0
000110758 0247_ $$2doi$$a10.1016/j.biomaterials.2020.119909
000110758 0248_ $$2sideral$$a117173
000110758 037__ $$aART-2020-117173
000110758 041__ $$aeng
000110758 100__ $$aSanchez-Casanova, Silvia
000110758 245__ $$aLocal delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regeneration
000110758 260__ $$c2020
000110758 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110758 5203_ $$aAchievement of spatiotemporal control of growth factors production remains a main goal in tissue engineering. In the present work, we combined inducible transgene expression and near infrared (NIR)-responsive hydrogels technologies to develop a therapeutic platform for bone regeneration. A heat-activated and dimerizer-dependent transgene expression system was incorporated into mesenchymal stem cells to conditionally control the production of bone morphogenetic protein 2 (BMP-2). Genetically engineered cells were entrapped in hydrogels based on fibrin and plasmonic gold nanoparticles that transduced incident energy of an NIR laser into heat. In the presence of dimerizer, photoinduced mild hyperthermia induced the release of bioactive BMP-2 from NIR-responsive cell constructs. A critical size bone defect, created in calvaria of immunocompetent mice, was filled with NIR-responsive hydrogels entrapping cells that expressed BMP-2 under the control of the heat-activated and dimerizer-dependent gene circuit. In animals that were treated with dimerizer, NIR irradiation of implants induced BMP-2 production in the bone lesion. Induction of NIR-responsive cell constructs conditionally expressing BMP-2 in bone defects resulted in the formation of new mineralized tissue, thus indicating the therapeutic potential of the technological platform.
000110758 536__ $$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM$$9info:eu-repo/grantAgreement/ES/ISCIII/PI15-01118$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-095159-B-100
000110758 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000110758 590__ $$a12.479$$b2020
000110758 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b2 / 40 = 0.05$$c2020$$dQ1$$eT1
000110758 591__ $$aENGINEERING, BIOMEDICAL$$b3 / 90 = 0.033$$c2020$$dQ1$$eT1
000110758 592__ $$a3.209$$b2020
000110758 593__ $$aBioengineering$$c2020$$dQ1
000110758 593__ $$aBiomaterials$$c2020$$dQ1
000110758 593__ $$aNanoscience and Nanotechnology$$c2020$$dQ1
000110758 593__ $$aCeramics and Composites$$c2020$$dQ1
000110758 593__ $$aMechanics of Materials$$c2020$$dQ1
000110758 593__ $$aBiophysics$$c2020$$dQ1
000110758 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000110758 700__ $$aMartin-Saavedra, Francisco M.
000110758 700__ $$aEscudero-Duch, Clara
000110758 700__ $$aFalguera Uceda, María I.
000110758 700__ $$0(orcid)0000-0002-2576-6382$$aPrieto, Martín
000110758 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, Manuel$$uUniversidad de Zaragoza
000110758 700__ $$aAcebo, Paloma
000110758 700__ $$aFabiilli, Mario L.
000110758 700__ $$aFranceschi, Renny T.
000110758 700__ $$aVilaboa, Nuria
000110758 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000110758 773__ $$g241 (2020), 119909 1-15$$pBiomaterials$$tBiomaterials$$x0142-9612
000110758 8564_ $$s205493$$uhttps://zaguan.unizar.es/record/110758/files/texto_completo.pdf$$yPostprint
000110758 8564_ $$s1565434$$uhttps://zaguan.unizar.es/record/110758/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000110758 909CO $$ooai:zaguan.unizar.es:110758$$particulos$$pdriver
000110758 951__ $$a2022-02-23-11:08:07
000110758 980__ $$aARTICLE