110758 20220223124038.0 doi 10.1016/j.biomaterials.2020.119909 sideral 117173 ART-2020-117173 eng Sanchez-Casanova, Silvia Local delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regeneration 2020 Achievement of spatiotemporal control of growth factors production remains a main goal in tissue engineering. In the present work, we combined inducible transgene expression and near infrared (NIR)-responsive hydrogels technologies to develop a therapeutic platform for bone regeneration. A heat-activated and dimerizer-dependent transgene expression system was incorporated into mesenchymal stem cells to conditionally control the production of bone morphogenetic protein 2 (BMP-2). Genetically engineered cells were entrapped in hydrogels based on fibrin and plasmonic gold nanoparticles that transduced incident energy of an NIR laser into heat. In the presence of dimerizer, photoinduced mild hyperthermia induced the release of bioactive BMP-2 from NIR-responsive cell constructs. A critical size bone defect, created in calvaria of immunocompetent mice, was filled with NIR-responsive hydrogels entrapping cells that expressed BMP-2 under the control of the heat-activated and dimerizer-dependent gene circuit. In animals that were treated with dimerizer, NIR irradiation of implants induced BMP-2 production in the bone lesion. Induction of NIR-responsive cell constructs conditionally expressing BMP-2 in bone defects resulted in the formation of new mineralized tissue, thus indicating the therapeutic potential of the technological platform. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/grantAgreement/ES/ISCIII/PI15-01118 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-095159-B-100 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 12.479 2020 MATERIALS SCIENCE, BIOMATERIALS 2 / 40 = 0.05 2020 Q1 T1 ENGINEERING, BIOMEDICAL 3 / 90 = 0.033 2020 Q1 T1 3.209 2020 Bioengineering 2020 Q1 Biomaterials 2020 Q1 Nanoscience and Nanotechnology 2020 Q1 Ceramics and Composites 2020 Q1 Mechanics of Materials 2020 Q1 Biophysics 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Martin-Saavedra, Francisco M. Escudero-Duch, Clara Falguera Uceda, María I. Prieto, Martín (orcid)0000-0002-2576-6382 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Acebo, Paloma Fabiilli, Mario L. Franceschi, Renny T. Vilaboa, Nuria 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 241 (2020), 119909 1-15 Biomaterials Biomaterials 0142-9612 205493 http://zaguan.unizar.es/record/110758/files/texto_completo.pdf Postprint 1565434 http://zaguan.unizar.es/record/110758/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:110758 articulos driver 2022-02-23-11:08:07 ARTICLE