000110774 001__ 110774
000110774 005__ 20240319080947.0
000110774 0247_ $$2doi$$a10.1007/s00248-021-01929-y
000110774 0248_ $$2sideral$$a125715
000110774 037__ $$aART-2022-125715
000110774 041__ $$aeng
000110774 100__ $$aWu-Chuang, Alejandra
000110774 245__ $$aThermostable Keystone bacteria maintain the functional diversity of the Ixodes scapularis microbiome under heat stress
000110774 260__ $$c2022
000110774 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110774 5203_ $$aVariations in the composition and diversity of tick microbiome due to high temperatures may influence the hierarchy of community members as a response to environmental change. Modifications in the community structure are hypothesized to drive alterations in the presence and/or abundance of functional pathways in the bacterial metagenome. In this study, this hypothesis was tested by using published 16S rRNA datasets of Ixodes scapularis males incubated at different temperatures (i.e., 4, 20, 30, and 37 degrees C) in a laboratory setting. Changes in community structure and functional profiles in response to temperature shifts were measured using co-occurrence networks and metagenome inference. Results from laboratory-reared ticks were then compared with those of field-collected ticks. The results from laboratory-reared ticks showed that high temperature altered the structure of the microbial community and decreased the number of keystone taxa. Notably, four taxa were identified as keystone in all the temperatures, and the functional diversity of the tick microbiome was contained in the four thermostable keystone their associated bacterial taxa. Three of the thermostable keystone taxa were also found in free-living ticks collected in Massachusetts. Moreover, the comparison of functional profiles of laboratory-reared and field-collected ticks revealed the existence of an important set of metabolic pathways that were common among the different datasets. Similar to the laboratory-reared ticks, the keystone taxa identified in field-collected ticks alongside their consortia (co-occurring taxa) were sufficient to retain the majority of the metabolic pathways in the functional profile. These results suggest that keystone taxa are essential in the stability and the functional resiliency of the tick microbiome under heat stress.
000110774 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000110774 590__ $$a3.6$$b2022
000110774 591__ $$aMARINE & FRESHWATER BIOLOGY$$b10 / 109 = 0.092$$c2022$$dQ1$$eT1
000110774 591__ $$aMICROBIOLOGY$$b65 / 135 = 0.481$$c2022$$dQ2$$eT2
000110774 591__ $$aECOLOGY$$b52 / 171 = 0.304$$c2022$$dQ2$$eT1
000110774 594__ $$a8.3$$b2022
000110774 592__ $$a1.072$$b2022
000110774 593__ $$aEcology$$c2022$$dQ1
000110774 593__ $$aSoil Science$$c2022$$dQ1
000110774 593__ $$aEcology, Evolution, Behavior and Systematics$$c2022$$dQ1
000110774 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000110774 700__ $$aObregon, Dasiel
000110774 700__ $$0(orcid)0000-0001-7483-046X$$aEstrada-Peña, Agustín$$uUniversidad de Zaragoza
000110774 700__ $$aCabezas-Cruz, Alejandro
000110774 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000110774 773__ $$g84 (2022), 1224–1235$$pMicrob. ecol.$$tMICROBIAL ECOLOGY$$x0095-3628
000110774 8564_ $$s3534482$$uhttps://zaguan.unizar.es/record/110774/files/texto_completo.pdf$$yVersión publicada
000110774 8564_ $$s2642092$$uhttps://zaguan.unizar.es/record/110774/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000110774 909CO $$ooai:zaguan.unizar.es:110774$$particulos$$pdriver
000110774 951__ $$a2024-03-18-12:40:37
000110774 980__ $$aARTICLE