110788 20240319080948.0 doi 10.3390/biology11020261 sideral 127642 ART-2022-127642 eng Carvajal Serna, M. Universidad de Zaragoza (orcid)0000-0003-3929-5064 Testicular ultrasound analysis as a predictive tool of ram sperm quality 2022 Access copy available to the general public Unrestricted esticular ultrasound is a non-invasive technique that could be very useful for predicting ram seminal quality. Recent software developments allow macroscopic and microscopic evaluation of testicular parenchyma. Thus, the aim of this study was to evaluate the testicular echotexture using ultrasound-video analysis and investigate its possible correlation with semen quality. Nine rams were evaluated for one year using a portable ultrasound scanner and the echotexture was analyzed with ECOTEXT® software. The number of black (Ec1), white (Ec2), and grey pixels (Ec3), tubular density (TD), lumen area (LA), and lumen diameter (LD) were analyzed. Semen was collected by an artificial vagina the same day and the sperm concentration, morphology, motility, viability, phosphatidylserine (PS) translocation, reactive-oxygen-species (ROS) levels, DNA damage and capacitation state were evaluated. Ec2 and Ec3 correlated positively with “bad quality” sperm parameters (the percentage of spermatozoa with high ROS levels, with PS translocation and proximal cytoplasmic droplets), and negatively with motility. In contrast, TD and LA showed a positive correlation with “good quality” parameters (motility or normal morphology) and a negative correlation with spermatozoa with high ROS levels, with DNA fragmentation, and proximal or distal cytoplasmic droplets. Thus, echotexture analysis by ultrasound-video analysis could be a valuable tool for assessing ram fertility. info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2017-83799-R info:eu-repo/grantAgreement/ES/DGA/A07-17R info:eu-repo/grantAgreement/ES/MICINN/PRE2018-085198 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.2 2022 BIOLOGY 25 / 92 = 0.272 2022 Q2 T1 0.779 2022 Agricultural and Biological Sciences (miscellaneous) 2022 Q1 Immunology and Microbiology (miscellaneous) 2022 Q2 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2022 Q2 4.0 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Miguel Jiménez, S. Universidad de Zaragoza (orcid)0000-0001-6733-9373 Pérez Pe, R. Universidad de Zaragoza (orcid)0000-0002-2312-6402 Casao, A. Universidad de Zaragoza (orcid)0000-0003-1997-4262 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 11, 2 (2022), 261 [18 pp.] Biology 2079-7737 971625 http://zaguan.unizar.es/record/110788/files/texto_completo.pdf Versión publicada 2609058 http://zaguan.unizar.es/record/110788/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:110788 articulos driver 2024-03-18-12:47:57 ARTICLE