000110819 001__ 110819
000110819 005__ 20240319080953.0
000110819 0247_ $$2doi$$a10.3390/ani12030251
000110819 0248_ $$2sideral$$a127580
000110819 037__ $$aART-2022-127580
000110819 041__ $$aeng
000110819 100__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, Luis V.$$uUniversidad de Zaragoza
000110819 245__ $$aOccurrence of rotavirus a genotypes and other enteric pathogens in diarrheic suckling piglets from Spanish swine farms
000110819 260__ $$c2022
000110819 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110819 5203_ $$aSpecies A rotavirus (RVA) is a major viral pathogen causing diarrhea in suckling piglets. Studies on its genetic heterogeneity have implications for vaccine efficacy in the field. In this study, fecal samples (n = 866) from diarrheic piglets younger than 28 days were analyzed over a two-year period (2018–2019). Samples were submitted from 426 farms located in 36 provinces throughout Spain and were tested using real-time PCR (qPCR) and reverse transcription real-time PCR (RT-qPCR) for five enteric pathogens. The individual prevalence was 89.4%, 64.4%, 44.9%, 33.7% and 4.4% for Clostridiumperfringens, Clostridioides (formerly Clostridium) difficile, species A rotavirus, species C rotavirus and porcine epidemic diarrhea virus, respectively. Most specimens (96.9%) were positive for at least one of the target pathogens, and more than 80% of samples harbored mixed infections. Nucleotide sequencing of 70 specimens positive for RVA revealed the presence of the VP7 genotypes G4, G9, G3, G5, G11 and the VP4 genotypes P7, P23, P6 and P13, with the combinations G4P7 and G9P23 being the most prevalent, and especially in the areas with the highest pig population. The study shows the extensive genetic diversity of RVA strains as well as discrepancies with the genotypes contained in the vaccine available in Spain, and multiple amino acid differences in antigenic epitopes of different G- and P- genotypes with the vaccine strains. Further investigations are needed to determine the efficacy of the vaccine to confer clinical protection against heterologous strains.
000110819 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A16-20R
000110819 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000110819 590__ $$a3.0$$b2022
000110819 592__ $$a0.684$$b2022
000110819 591__ $$aVETERINARY SCIENCES$$b13 / 144 = 0.09$$c2022$$dQ1$$eT1
000110819 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000110819 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b12 / 62 = 0.194$$c2022$$dQ1$$eT1
000110819 593__ $$aAnimal Science and Zoology$$c2022$$dQ1
000110819 594__ $$a4.2$$b2022
000110819 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000110819 700__ $$0(orcid)0000-0002-2497-9028$$aBenito, Alfredo A.
000110819 700__ $$aLázaro-Gaspar, Sofía
000110819 700__ $$aArnal, José L.
000110819 700__ $$aMartín-Jurado, Desirée
000110819 700__ $$aMenjon, Rut
000110819 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, Joaquín$$uUniversidad de Zaragoza
000110819 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000110819 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000110819 773__ $$g12, 3 (2022), 251 [16 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000110819 8564_ $$s1358389$$uhttps://zaguan.unizar.es/record/110819/files/texto_completo.pdf$$yVersión publicada
000110819 8564_ $$s2919200$$uhttps://zaguan.unizar.es/record/110819/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000110819 909CO $$ooai:zaguan.unizar.es:110819$$particulos$$pdriver
000110819 951__ $$a2024-03-18-13:17:30
000110819 980__ $$aARTICLE