000110827 001__ 110827
000110827 005__ 20230519145619.0
000110827 0247_ $$2doi$$a10.1002/mbo3.1235
000110827 0248_ $$2sideral$$a127482
000110827 037__ $$aART-2021-127482
000110827 041__ $$aeng
000110827 100__ $$aRomán, F.
000110827 245__ $$aCharacterization of methicillin-resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children
000110827 260__ $$c2021
000110827 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110827 5203_ $$aObjective: To characterize the Staphylococcus aureus strains colonizing healthy Spanish children. Methods: Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton-Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin-resistant Staphylococcus aureus (MRSA) strains, as well as pulsed-field gel electrophoresis (PFGE). Results: 619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA-positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene. Conclusions: methicillin-resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst.
000110827 536__ $$9info:eu-repo/grantAgreement/ES/MICINN-ISCIII-FEDER-FIS/PI18CIII-00372
000110827 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000110827 590__ $$a3.904$$b2021
000110827 592__ $$a0.711$$b2021
000110827 594__ $$a5.9$$b2021
000110827 591__ $$aMICROBIOLOGY$$b73 / 138 = 0.529$$c2021$$dQ3$$eT2
000110827 593__ $$aMicrobiology$$c2021$$dQ3
000110827 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000110827 700__ $$aMendez-Echevarria, A.
000110827 700__ $$aDel Rosal, T.
000110827 700__ $$0(orcid)0000-0002-8459-9705$$aGarcia-Vera, C.$$uUniversidad de Zaragoza
000110827 700__ $$aEscosa-Garcia, L.
000110827 700__ $$aAgud, M.
000110827 700__ $$aChaves, F.
000110827 700__ $$aGutiérrez-Fernández, J.
000110827 700__ $$aRuiz de Gopegui, E.
000110827 700__ $$aRuiz-Carrascoso, G.
000110827 700__ $$aRuiz-Gallego, M. C.
000110827 700__ $$aBernet A.
000110827 700__ $$aQuevedo S. M.
000110827 700__ $$aFernández-Verdugo, A. M.
000110827 700__ $$aSainz, T.
000110827 700__ $$aCalvo, C.
000110827 7102_ $$11011$$2670$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Pediatría
000110827 773__ $$g10, 5 (2021), e1235 [11 pp.]$$pMicrobiologyOpen$$tMicrobiologyOpen$$x2045-8827
000110827 8564_ $$s780213$$uhttps://zaguan.unizar.es/record/110827/files/texto_completo.pdf$$yVersión publicada
000110827 8564_ $$s2262981$$uhttps://zaguan.unizar.es/record/110827/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000110827 909CO $$ooai:zaguan.unizar.es:110827$$particulos$$pdriver
000110827 951__ $$a2023-05-18-16:13:13
000110827 980__ $$aARTICLE