000110881 001__ 110881
000110881 005__ 20240319081000.0
000110881 0247_ $$2doi$$a10.3390/nano12050755
000110881 0248_ $$2sideral$$a127721
000110881 037__ $$aART-2022-127721
000110881 041__ $$aeng
000110881 100__ $$0(orcid)0000-0002-1791-0188$$aBonet-Aleta, Javier$$uUniversidad de Zaragoza
000110881 245__ $$aGold-Platinum Nanoparticles with Core-Shell Configuration as Efficient Oxidase-like Nanosensors for Glutathione Detection
000110881 260__ $$c2022
000110881 5060_ $$aAccess copy available to the general public$$fUnrestricted
000110881 5203_ $$aNanozymes, defined as nanomaterials that can mimic the catalytic activity of natural enzymes, have been widely used to develop analytical tools for biosensing. In this regard, the monitoring of glutathione (GSH), a key antioxidant biomolecule intervening in the regulation of the oxidative stress level of cells or related with Parkinson’s or mitochondrial diseases can be of great interest from the biomedical point of view. In this work, we have synthetized a gold-platinum Au@Pt nanoparticle with core-shell configuration exhibiting a remarkable oxidase-like mimicking activity towards the substrates 3,3′,5,5′-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD). The presence of a thiol group (-SH) in the chemical structure of GSH can bind to the Au@Pt nanozyme surface to hamper the activation of O2 and reducing its oxidase-like activity as a function of the concentration of GSH. Herein, we exploit the loss of activity to develop an analytical methodology able to detect and quantify GSH up to µM levels. The system composed by Au@Pt and TMB demonstrates a good linear range between 0.1–1.0 µM to detect GSH levels with a limit of detection (LoD) of 34 nM.
000110881 536__ $$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE$$9info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE$$9info:eu-repo/grantAgreement/ES/MCIU/FPU18/04618
000110881 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000110881 590__ $$a5.3$$b2022
000110881 592__ $$a0.811$$b2022
000110881 591__ $$aPHYSICS, APPLIED$$b39 / 160 = 0.244$$c2022$$dQ1$$eT1
000110881 593__ $$aChemical Engineering (miscellaneous)$$c2022$$dQ1
000110881 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b51 / 107 = 0.477$$c2022$$dQ2$$eT2
000110881 593__ $$aMaterials Science (miscellaneous)$$c2022$$dQ2
000110881 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b58 / 178 = 0.326$$c2022$$dQ2$$eT1
000110881 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b110 / 343 = 0.321$$c2022$$dQ2$$eT1
000110881 594__ $$a7.4$$b2022
000110881 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000110881 700__ $$0(orcid)0000-0003-4874-6672$$aGarcia-Peiro, Jose I.$$uUniversidad de Zaragoza
000110881 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, S.$$uUniversidad de Zaragoza
000110881 700__ $$0(orcid)0000-0002-4546-4111$$aHueso, Jose L.$$uUniversidad de Zaragoza
000110881 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000110881 7102_ $$15005$$2790$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Tecnologi. Medio Ambiente
000110881 773__ $$g12 (2022), 755 [13 pp.]$$pNanomaterials (Basel)$$tNanomaterials$$x2079-4991
000110881 8564_ $$s2789828$$uhttps://zaguan.unizar.es/record/110881/files/texto_completo.pdf$$yVersión publicada
000110881 8564_ $$s2833513$$uhttps://zaguan.unizar.es/record/110881/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000110881 909CO $$ooai:zaguan.unizar.es:110881$$particulos$$pdriver
000110881 951__ $$a2024-03-18-14:04:25
000110881 980__ $$aARTICLE