110881 20240319081000.0 doi 10.3390/nano12050755 sideral 127721 ART-2022-127721 eng Bonet-Aleta, Javier Universidad de Zaragoza (orcid)0000-0002-1791-0188 Gold-Platinum Nanoparticles with Core-Shell Configuration as Efficient Oxidase-like Nanosensors for Glutathione Detection 2022 Access copy available to the general public Unrestricted Nanozymes, defined as nanomaterials that can mimic the catalytic activity of natural enzymes, have been widely used to develop analytical tools for biosensing. In this regard, the monitoring of glutathione (GSH), a key antioxidant biomolecule intervening in the regulation of the oxidative stress level of cells or related with Parkinson’s or mitochondrial diseases can be of great interest from the biomedical point of view. In this work, we have synthetized a gold-platinum Au@Pt nanoparticle with core-shell configuration exhibiting a remarkable oxidase-like mimicking activity towards the substrates 3,3′,5,5′-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD). The presence of a thiol group (-SH) in the chemical structure of GSH can bind to the Au@Pt nanozyme surface to hamper the activation of O2 and reducing its oxidase-like activity as a function of the concentration of GSH. Herein, we exploit the loss of activity to develop an analytical methodology able to detect and quantify GSH up to µM levels. The system composed by Au@Pt and TMB demonstrates a good linear range between 0.1–1.0 µM to detect GSH levels with a limit of detection (LoD) of 34 nM. info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/grantAgreement/ES/MCIU/FPU18/04618 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.3 2022 PHYSICS, APPLIED 39 / 160 = 0.244 2022 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 51 / 107 = 0.477 2022 Q2 T2 CHEMISTRY, MULTIDISCIPLINARY 58 / 178 = 0.326 2022 Q2 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 110 / 343 = 0.321 2022 Q2 T1 0.811 2022 Chemical Engineering (miscellaneous) 2022 Q1 Materials Science (miscellaneous) 2022 Q2 7.4 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Garcia-Peiro, Jose I. Universidad de Zaragoza (orcid)0000-0003-4874-6672 Irusta, S. Universidad de Zaragoza (orcid)0000-0002-2966-9088 Hueso, Jose L. Universidad de Zaragoza (orcid)0000-0002-4546-4111 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 12 (2022), 755 [13 pp.] Nanomaterials (Basel) Nanomaterials 2079-4991 2789828 http://zaguan.unizar.es/record/110881/files/texto_completo.pdf Versión publicada 2833513 http://zaguan.unizar.es/record/110881/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:110881 articulos driver 2024-03-18-14:04:25 ARTICLE