000112013 001__ 112013
000112013 005__ 20240319080949.0
000112013 0247_ $$2doi$$a10.3390/ijms23073579
000112013 0248_ $$2sideral$$a127945
000112013 037__ $$aART-2022-127945
000112013 041__ $$aeng
000112013 100__ $$0(orcid)0000-0002-8752-5146$$aGarcía-Martínez, Mirta$$uUniversidad de Zaragoza
000112013 245__ $$aDistinctive Toll-like Receptors Gene Expression and Glial Response in Different Brain Regions of Natural Scrapie
000112013 260__ $$c2022
000112013 5060_ $$aAccess copy available to the general public$$fUnrestricted
000112013 5203_ $$aPrion diseases are chronic and fatal neurodegenerative diseases characterized by the accumulation of disease-specific prion protein (PrPSc), spongiform changes, neuronal loss, and gliosis. Growing evidence shows that the neuroinflammatory response is a key component of prion diseases and contributes to neurodegeneration. Toll-like receptors (TLRs) have been proposed as important mediators of innate immune responses triggered in the central nervous system in other human neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. However, little is known about the role of TLRs in prion diseases, and their involvement in the neuropathology of natural scrapie has not been studied. We assessed the gene expression of ovine TLRs in four anatomically distinct brain regions in natural scrapie-infected sheep and evaluated the possible correlations between gene expression and the pathological hallmarks of prion disease. We observed significant changes in TLR expression in scrapie-infected sheep that correlate with the degree of spongiosis, PrPSc deposition, and gliosis in each of the regions studied. Remarkably, TLR4 was the only gene upregulated in all regions, regardless of the severity of neuropathology. In the hippocampus, we observed milder neuropathology associated with a distinct TLR gene expression profile and the presence of a peculiar microglial morphology, called rod microglia, described here for the first time in the brain of scrapie-infected sheep. The concurrence of these features suggests partial neuroprotection of the hippocampus. Finally, a comparison of the findings in naturallyinfected sheep versus an ovinized mouse model (tg338 mice) revealed distinct patterns of TLRgene expression.
000112013 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A05-20R
000112013 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000112013 590__ $$a5.6$$b2022
000112013 592__ $$a1.154$$b2022
000112013 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000112013 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000112013 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000112013 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000112013 593__ $$aComputer Science Applications$$c2022$$dQ1
000112013 593__ $$aInorganic Chemistry$$c2022$$dQ1
000112013 593__ $$aSpectroscopy$$c2022$$dQ1
000112013 593__ $$aOrganic Chemistry$$c2022$$dQ1
000112013 593__ $$aMolecular Biology$$c2022$$dQ2
000112013 593__ $$aCatalysis$$c2022$$dQ2
000112013 594__ $$a7.8$$b2022
000112013 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000112013 700__ $$aCortez, Leonardo M.
000112013 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, Alicia$$uUniversidad de Zaragoza
000112013 700__ $$0(orcid)0000-0002-0388-9429$$aBetancor, Marina$$uUniversidad de Zaragoza
000112013 700__ $$aSerrano-Pérez, Beatriz
000112013 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000112013 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000112013 700__ $$0(orcid)0000-0001-7098-2327$$aGarza, María Carmen$$uUniversidad de Zaragoza
000112013 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000112013 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000112013 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000112013 773__ $$g23, 7 (2022), 3579 [24 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000112013 8564_ $$s2228290$$uhttps://zaguan.unizar.es/record/112013/files/texto_completo.pdf$$yVersión publicada
000112013 8564_ $$s2730165$$uhttps://zaguan.unizar.es/record/112013/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000112013 909CO $$ooai:zaguan.unizar.es:112013$$particulos$$pdriver
000112013 951__ $$a2024-03-18-12:53:38
000112013 980__ $$aARTICLE