000112052 001__ 112052 000112052 005__ 20230519145552.0 000112052 0247_ $$2doi$$a10.3390/antiox10071109 000112052 0248_ $$2sideral$$a126569 000112052 037__ $$aART-2021-126569 000112052 041__ $$aeng 000112052 100__ $$aCasula E. 000112052 245__ $$aNasal spray formulations based on combined hyalurosomes and glycerosomes loading zingiber officinalis extract as green and natural strategy for the treatment of rhinitis and rhinosinusitis 000112052 260__ $$c2021 000112052 5060_ $$aAccess copy available to the general public$$fUnrestricted 000112052 5203_ $$aA total green nanotechnological nasal spray has been manufactured and proposed as an alternative treatment of rhinitis and rhinosinusitis. It was obtained by combining the strengthening effect of liposomes on barrier function, the hydrating and lubricating properties of sodium hyaluro-nan and the anti-inflammatory and antioxidant activities of the extract of Zingiber officinalis. To this purpose, the extract was loaded in special phospholipid vesicles immobilized with hyaluronic acid (hyalurosomes), which were further enriched with glycerol in the water phase. Liposomes and glycerosomes were prepared as well and used as reference. Vesicles were oligolamellar and multi-compartment, as confirmed by cryogenic transmission electron microscopy (cryo-TEM) observation, small in size (~140 nm) and negatively charged (~-23 mV). Spray characteristics were evaluated by using the Spraytec® and instant images, from which the plume angle was measured. The range of the droplet size distribution and the narrow spray angle obtained suggest a good nebulization and a possible local deposition in the nasal cavity. In vitro studies performed by using human keratinocytes confirmed the high biocompatibility of vesicles and their ability to effectively counteract oxidative damage on cells induced by hydrogen peroxide. The overall collected data suggest that our vesicles are suitable as nasal spray. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. 000112052 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000112052 590__ $$a7.675$$b2021 000112052 592__ $$a1.008$$b2021 000112052 594__ $$a6.5$$b2021 000112052 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b50 / 297 = 0.168$$c2021$$dQ1$$eT1 000112052 593__ $$aBiochemistry$$c2021$$dQ1 000112052 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b12 / 144 = 0.083$$c2021$$dQ1$$eT1 000112052 593__ $$aPhysiology$$c2021$$dQ1 000112052 591__ $$aCHEMISTRY, MEDICINAL$$b4 / 63 = 0.063$$c2021$$dQ1$$eT1 000112052 593__ $$aMolecular Biology$$c2021$$dQ1 000112052 593__ $$aClinical Biochemistry$$c2021$$dQ1 000112052 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000112052 700__ $$aManca M.L. 000112052 700__ $$aPerra M. 000112052 700__ $$aPedraz J.L. 000112052 700__ $$aLopez-Mendez T.B. 000112052 700__ $$0(orcid)0000-0003-4141-6072$$aLozano Fantova A. 000112052 700__ $$0(orcid)0000-0002-5631-1401$$aCalvo E.$$uUniversidad de Zaragoza 000112052 700__ $$aZaru M. 000112052 700__ $$aManconi M. 000112052 7102_ $$15001$$2600$$aUniversidad de Zaragoza$$bDpto. Ciencia Tecnol.Mater.Fl.$$cÁrea Mecánica de Fluidos 000112052 773__ $$g10, 7 (2021), 1109 [16 pp]$$pAntioxidants$$tAntioxidants$$x2076-3921 000112052 8564_ $$s6265984$$uhttps://zaguan.unizar.es/record/112052/files/texto_completo.pdf$$yVersión publicada 000112052 8564_ $$s2770528$$uhttps://zaguan.unizar.es/record/112052/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000112052 909CO $$ooai:zaguan.unizar.es:112052$$particulos$$pdriver 000112052 951__ $$a2023-05-18-15:51:40 000112052 980__ $$aARTICLE