000112143 001__ 112143
000112143 005__ 20230519145429.0
000112143 0247_ $$2doi$$a10.1080/21505594.2021.2003115
000112143 0248_ $$2sideral$$a125696
000112143 037__ $$aART-2021-125696
000112143 041__ $$aeng
000112143 100__ $$0(orcid)0000-0002-8134-0693$$aArenas, Jesús$$uUniversidad de Zaragoza
000112143 245__ $$aSerum proteases prevent bacterial biofilm formation: role of kallikrein and plasmin
000112143 260__ $$c2021
000112143 5060_ $$aAccess copy available to the general public$$fUnrestricted
000112143 5203_ $$aBiofilm formation is a general strategy for bacterial pathogens to withstand host defense mechanisms. In this study, we found that serum proteases inhibit biofilm formation by Neisseria meningitidis, Neisseria gonorrhoeae, Haemophilus influenzae, and Bordetella pertussis. Confocal laser-scanning microscopy analysis revealed that these proteins reduce the biomass and alter the architecture of meningococcal biofilms. To understand the underlying mechanism, the serum was fractionated through size-exclusion chromatography and anion-exchange chromatography, and the composition of the fractions that retained anti-biofilm activity against N. meningitidis was analyzed by intensity-based absolute quantification mass spectrometry. Among the identified serum proteins, plasma kallikrein (PKLK), FXIIa, and plasmin were found to cleave neisserial heparin-binding antigen and the alpha-peptide of IgA protease on the meningococcal cell surface, resulting in the release of positively charged polypeptides implicated in biofilm formation by binding extracellular DNA. Further experiments also revealed that plasmin and PKLK inhibited biofilm formation of B. pertussis by cleaving filamentous hemagglutinin. We conclude that the proteolytic activity of serum proteases toward bacterial adhesins involved in biofilm formation could constitute a defense mechanism for the clearance of pathogens.
000112143 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000112143 590__ $$a5.428$$b2021
000112143 592__ $$a1.107$$b2021
000112143 594__ $$a7.1$$b2021
000112143 591__ $$aIMMUNOLOGY$$b65 / 163 = 0.399$$c2021$$dQ2$$eT2
000112143 593__ $$aImmunology$$c2021$$dQ1
000112143 591__ $$aMICROBIOLOGY$$b43 / 138 = 0.312$$c2021$$dQ2$$eT1
000112143 593__ $$aParasitology$$c2021$$dQ1
000112143 591__ $$aINFECTIOUS DISEASES$$b36 / 96 = 0.375$$c2021$$dQ2$$eT2
000112143 593__ $$aMicrobiology (medical)$$c2021$$dQ1
000112143 593__ $$aMicrobiology$$c2021$$dQ1
000112143 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000112143 700__ $$aSzabo, Zalan
000112143 700__ $$aWal, Jelle van der
000112143 700__ $$aMaas, Coen
000112143 700__ $$aRiaz, Tahira
000112143 700__ $$aTonjum, Tone
000112143 700__ $$aTommassen, Jan
000112143 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000112143 773__ $$g12, 1 (2021), 2902-2917$$pVIRULENCE$$tVirulence$$x2150-5594
000112143 8564_ $$s2290438$$uhttps://zaguan.unizar.es/record/112143/files/texto_completo.pdf$$yVersión publicada
000112143 8564_ $$s1034306$$uhttps://zaguan.unizar.es/record/112143/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000112143 909CO $$ooai:zaguan.unizar.es:112143$$particulos$$pdriver
000112143 951__ $$a2023-05-18-14:16:49
000112143 980__ $$aARTICLE