000112436 001__ 112436
000112436 005__ 20240319080959.0
000112436 0247_ $$2doi$$a10.3390/ani12080984
000112436 0248_ $$2sideral$$a128513
000112436 037__ $$aART-2022-128513
000112436 041__ $$aeng
000112436 100__ $$0(orcid)0000-0002-1481-9805$$aCequier, Alina$$uUniversidad de Zaragoza
000112436 245__ $$aEquine mesenchymal stem cells influence the proliferative response of lymphocytes: effect of inflammation, differentiation and MHC-compatibility
000112436 260__ $$c2022
000112436 5060_ $$aAccess copy available to the general public$$fUnrestricted
000112436 5203_ $$aImmunomodulation and immunogenicity are pivotal aspects for the therapeutic use of mesenchymal stem cells (MSCs). Since the horse is highly valuable as both a patient and translational model, further knowledge on equine MSC immune properties is required. This study analysed how inflammation, chondrogenic differentiation and compatibility for the major histocompatibility complex (MHC) influence the MSC immunomodulatory–immunogenicity balance. Equine MSCs in basal conditions, pro-inflammatory primed (MSC-primed) or chondrogenically differentiated (MSC-chondro) were co-cultured with either autologous or allogeneic MHC-matched/mismatched lymphocytes in immune-suppressive assays (immunomodulation) and in modified one-way mixed leukocyte reactions (immunogenicity). After co-culture, frequency and proliferation of T cell subsets and B cells were assessed by flow cytometry and interferon-ɣ (IFNɣ) secretion by ELISA. MSC-primed showed higher regulatory potential by decreasing proliferation of cytotoxic and helper T cells and B cells. However, MHC-mismatched MSC-primed can also activate lymphocytes (proliferative response and IFNɣ secretion), likely due to increased MHC-expression. MSC-chondro maintained their regulatory ability and did not increase their immunogenicity, but showed less capacity than MSC-primed to induce regulatory T cells and further stimulated B cells. Subsequent in vivo studies are needed to elucidate the complex interactions between MSCs and the recipient immune system, which is critical to develop safe and effective therapies.
000112436 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/A19-17R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-84411-P
000112436 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000112436 590__ $$a3.0$$b2022
000112436 592__ $$a0.684$$b2022
000112436 591__ $$aVETERINARY SCIENCES$$b13 / 144 = 0.09$$c2022$$dQ1$$eT1
000112436 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000112436 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b12 / 62 = 0.194$$c2022$$dQ1$$eT1
000112436 593__ $$aAnimal Science and Zoology$$c2022$$dQ1
000112436 594__ $$a4.2$$b2022
000112436 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000112436 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, Antonio$$uUniversidad de Zaragoza
000112436 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, Francisco J.$$uUniversidad de Zaragoza
000112436 700__ $$0(orcid)0000-0003-1286-4968$$aVitoria, Arantza$$uUniversidad de Zaragoza
000112436 700__ $$0(orcid)0000-0002-9710-0395$$aBernad, Elvira$$uUniversidad de Zaragoza
000112436 700__ $$aFuente, Sara$$uUniversidad de Zaragoza
000112436 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000112436 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, Clementina$$uUniversidad de Zaragoza
000112436 700__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, Laura$$uUniversidad de Zaragoza
000112436 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000112436 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000112436 773__ $$g12, 8 (2022), 984 [25 pp.]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000112436 8564_ $$s2259351$$uhttps://zaguan.unizar.es/record/112436/files/texto_completo.pdf$$yVersión publicada
000112436 8564_ $$s2886488$$uhttps://zaguan.unizar.es/record/112436/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000112436 909CO $$ooai:zaguan.unizar.es:112436$$particulos$$pdriver
000112436 951__ $$a2024-03-18-13:57:42
000112436 980__ $$aARTICLE