112436 20240319080959.0 doi 10.3390/ani12080984 sideral 128513 ART-2022-128513 eng Cequier, Alina Universidad de Zaragoza (orcid)0000-0002-1481-9805 Equine mesenchymal stem cells influence the proliferative response of lymphocytes: effect of inflammation, differentiation and MHC-compatibility 2022 Access copy available to the general public Unrestricted Immunomodulation and immunogenicity are pivotal aspects for the therapeutic use of mesenchymal stem cells (MSCs). Since the horse is highly valuable as both a patient and translational model, further knowledge on equine MSC immune properties is required. This study analysed how inflammation, chondrogenic differentiation and compatibility for the major histocompatibility complex (MHC) influence the MSC immunomodulatory–immunogenicity balance. Equine MSCs in basal conditions, pro-inflammatory primed (MSC-primed) or chondrogenically differentiated (MSC-chondro) were co-cultured with either autologous or allogeneic MHC-matched/mismatched lymphocytes in immune-suppressive assays (immunomodulation) and in modified one-way mixed leukocyte reactions (immunogenicity). After co-culture, frequency and proliferation of T cell subsets and B cells were assessed by flow cytometry and interferon-ɣ (IFNɣ) secretion by ELISA. MSC-primed showed higher regulatory potential by decreasing proliferation of cytotoxic and helper T cells and B cells. However, MHC-mismatched MSC-primed can also activate lymphocytes (proliferative response and IFNɣ secretion), likely due to increased MHC-expression. MSC-chondro maintained their regulatory ability and did not increase their immunogenicity, but showed less capacity than MSC-primed to induce regulatory T cells and further stimulated B cells. Subsequent in vivo studies are needed to elucidate the complex interactions between MSCs and the recipient immune system, which is critical to develop safe and effective therapies. info:eu-repo/grantAgreement/ES/DGA-FSE/A19-17R info:eu-repo/grantAgreement/ES/MINECO/AGL2017-84411-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.0 2022 VETERINARY SCIENCES 13 / 144 = 0.09 2022 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 12 / 62 = 0.194 2022 Q1 T1 0.684 2022 Veterinary (miscellaneous) 2022 Q1 Animal Science and Zoology 2022 Q1 4.2 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Romero, Antonio Universidad de Zaragoza (orcid)0000-0001-7188-0461 Vázquez, Francisco J. Universidad de Zaragoza (orcid)0000-0002-8712-2275 Vitoria, Arantza Universidad de Zaragoza (orcid)0000-0003-1286-4968 Bernad, Elvira Universidad de Zaragoza (orcid)0000-0002-9710-0395 Fuente, Sara Universidad de Zaragoza Zaragoza, Pilar Universidad de Zaragoza (orcid)0000-0001-5740-0185 Rodellar, Clementina Universidad de Zaragoza (orcid)0000-0003-3289-2675 Barrachina, Laura Universidad de Zaragoza (orcid)0000-0001-9818-508X 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 12, 8 (2022), 984 [25 pp.] Animals (Basel) Animals 2076-2615 2259351 http://zaguan.unizar.es/record/112436/files/texto_completo.pdf Versión publicada 2886488 http://zaguan.unizar.es/record/112436/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:112436 articulos driver 2024-03-18-13:57:42 ARTICLE