000112444 001__ 112444
000112444 005__ 20240219135521.0
000112444 0247_ $$2doi$$a10.1063/5.0016374
000112444 0248_ $$2sideral$$a122534
000112444 037__ $$aART-2020-122534
000112444 041__ $$aeng
000112444 100__ $$aSalvador-Clavell, R.
000112444 245__ $$aDesign and experimental validation of a magnetic device for stem cell culture
000112444 260__ $$c2020
000112444 5060_ $$aAccess copy available to the general public$$fUnrestricted
000112444 5203_ $$aCell culture of bone and tendon tissues requires mechanical stimulation of the cells in order to mimic their physiological state. In the present work, a device has been conceived and developed to generate a controlled magnetic field with a homogeneous gradient in the working space. The design requirement was to maximize the magnetic flux gradient, assuring a minimum magnetizing value in a 15 mm × 15 mm working area, which highly increases the normal operating range of this sort of devices. The objective is to use the machine for two types of biological tests: magnetic irradiation of biological samples and force generation on paramagnetic particles embedded in scaffolds for cell culture. The device has been manufactured and experimentally validated by evaluating the force exerted on magnetic particles in a viscous fluid. Apart from the magnetic validation, the device has been tested for irradiating biological samples. In this case, viability of human dental pulp stem cells has been studied in vitro after electromagnetic field exposition using the designed device. After three days of irradiation treatment, cellular microtissues showed a 59% increase in the viable cell number. Irradiated cells did not show morphological differences when compared with control cells.
000112444 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/MAT2016-76039-C4-4-R$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2016-76039-C4-2-R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-106099RB-C44$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-106099RB-C42-AEI-10.13039-501100011033$$9info:eu-repo/grantAgreement/ES/DGA-FSE/T24-20R
000112444 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000112444 590__ $$a1.523$$b2020
000112444 591__ $$aINSTRUMENTS & INSTRUMENTATION$$b46 / 64 = 0.719$$c2020$$dQ3$$eT3
000112444 591__ $$aPHYSICS, APPLIED$$b124 / 160 = 0.775$$c2020$$dQ4$$eT3
000112444 592__ $$a0.604$$b2020
000112444 593__ $$aMedicine (miscellaneous)$$c2020$$dQ2
000112444 593__ $$aInstrumentation$$c2020$$dQ2
000112444 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000112444 700__ $$aRodríguez-Fortún, J.M.$$uUniversidad de Zaragoza
000112444 700__ $$aLópez, I.
000112444 700__ $$aMartín De Llano, J.J.
000112444 700__ $$aOrús, J.
000112444 700__ $$aSancho-Tello, M.
000112444 700__ $$aCarda, C.
000112444 700__ $$0(orcid)0000-0003-0088-7222$$aHamdy Doweidar, M.$$uUniversidad de Zaragoza
000112444 7102_ $$15007$$2520$$aUniversidad de Zaragoza$$bDpto. Informát.Ingenie.Sistms.$$cÁrea Ingen.Sistemas y Automát.
000112444 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000112444 773__ $$g91, 12 (2020), 124103 [9 pp.]$$pRev. sci. instrum.$$tReview of Scientific Instruments$$x0034-6748
000112444 8564_ $$s1164876$$uhttps://zaguan.unizar.es/record/112444/files/texto_completo.pdf$$yPostprint
000112444 8564_ $$s1463113$$uhttps://zaguan.unizar.es/record/112444/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000112444 909CO $$ooai:zaguan.unizar.es:112444$$particulos$$pdriver
000112444 951__ $$a2024-02-19-13:51:39
000112444 980__ $$aARTICLE