112444 20240219135521.0 doi 10.1063/5.0016374 sideral 122534 ART-2020-122534 eng Salvador-Clavell, R. Design and experimental validation of a magnetic device for stem cell culture 2020 Access copy available to the general public Unrestricted Cell culture of bone and tendon tissues requires mechanical stimulation of the cells in order to mimic their physiological state. In the present work, a device has been conceived and developed to generate a controlled magnetic field with a homogeneous gradient in the working space. The design requirement was to maximize the magnetic flux gradient, assuring a minimum magnetizing value in a 15 mm × 15 mm working area, which highly increases the normal operating range of this sort of devices. The objective is to use the machine for two types of biological tests: magnetic irradiation of biological samples and force generation on paramagnetic particles embedded in scaffolds for cell culture. The device has been manufactured and experimentally validated by evaluating the force exerted on magnetic particles in a viscous fluid. Apart from the magnetic validation, the device has been tested for irradiating biological samples. In this case, viability of human dental pulp stem cells has been studied in vitro after electromagnetic field exposition using the designed device. After three days of irradiation treatment, cellular microtissues showed a 59% increase in the viable cell number. Irradiated cells did not show morphological differences when compared with control cells. info:eu-repo/grantAgreement/ES/DGA-FSE/T24-20R info:eu-repo/grantAgreement/ES/MICINN/PID2019-106099RB-C42-AEI-10.13039-501100011033 info:eu-repo/grantAgreement/ES/MICINN/PID2019-106099RB-C44 info:eu-repo/grantAgreement/ES/MINECO/MAT2016-76039-C4-2-R info:eu-repo/grantAgreement/ES/MINECO/MAT2016-76039-C4-4-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.523 2020 INSTRUMENTS & INSTRUMENTATION 46 / 64 = 0.719 2020 Q3 T3 PHYSICS, APPLIED 124 / 160 = 0.775 2020 Q4 T3 0.604 2020 Medicine (miscellaneous) 2020 Q2 Instrumentation 2020 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Rodríguez-Fortún, J.M. Universidad de Zaragoza López, I. Martín De Llano, J.J. Orús, J. Sancho-Tello, M. Carda, C. Hamdy Doweidar, M. Universidad de Zaragoza (orcid)0000-0003-0088-7222 5007 520 Universidad de Zaragoza Dpto. Informát.Ingenie.Sistms. Área Ingen.Sistemas y Automát. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 91, 12 (2020), 124103 [9 pp.] Rev. sci. instrum. Review of Scientific Instruments 0034-6748 1164876 http://zaguan.unizar.es/record/112444/files/texto_completo.pdf Postprint 1463113 http://zaguan.unizar.es/record/112444/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:112444 articulos driver 2024-02-19-13:51:39 ARTICLE