112455 20240319080951.0 doi 10.1016/j.talanta.2022.123226 sideral 128379 ART-2022-128379 eng Blázquez-García, Marina Ultrasensitive detection of soy traces by immunosensing of glycinin and ß-conglycinin at disposable electrochemical platforms 2022 Access copy available to the general public Unrestricted This work reports the first electrochemical bioplatform for the determination of soy traces in food. The bioplatform involves sandwich-type immunoassays using specific antibodies for ß-conglycinin and glycinin, which are the main allergenic soy proteins, and carboxylic acid-modified magnetic microbeads. Amperometric detection at -0.20 V (vs. an Ag pseudo-reference electrode) was performed using single or dual screen-printed carbon electrodes and the H2O2/hydroquinone (HQ) system. The measured variation in the cathodic current was directly proportional to the concentration of target allergenic proteins. The developed bioplatforms exhibit a good selectivity and sensitivity providing limits of detection (LOD) values of 0.03 and 0.02 ng mL-1 for ß-conglycinin and glycinin, respectively. The determination of both proteins can be carried out in only 1.5 h. The electrochemical bioplatforms allow their accurate determinations (with results statistically comparable to those provided by ELISA methodologies) in raw cookie dough and baked cookies enriched with soy flour. The results obtained confirm, in a pioneering way with electrochemical biosensors, the possibility of discriminating samples incurred with as little as 0.0005 ppm of a food allergen in model cookie extracts. info:eu-repo/grantAgreement/ES/MICINN/IJCI-2017-31345 info:eu-repo/grantAgreement/ES/MICINN/PID2019-103899RB-100 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.1 2022 CHEMISTRY, ANALYTICAL 9 / 86 = 0.105 2022 Q1 T1 0.986 2022 Analytical Chemistry 2022 Q1 Spectroscopy 2022 Q1 Biochemistry 2022 Q2 12.2 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Arévalo, Beatriz Serafín, Verónica Benedé, Sara Mata, Luis Galán-Malo, Patricia Segura-Gil, Isabel Pérez, María Dolores Universidad de Zaragoza (orcid)0000-0003-2555-8425 Pingarrón, José M. Campuzano, Susana 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 241 (2022), 123226 [7 pp] Talanta Talanta 0039-9140 3956566 http://zaguan.unizar.es/record/112455/files/texto_completo.pdf Versión publicada 2128080 http://zaguan.unizar.es/record/112455/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:112455 articulos driver 2024-03-18-13:05:33 ARTICLE