000112513 001__ 112513
000112513 005__ 20240319080954.0
000112513 0247_ $$2doi$$a10.3390/ijms23031150
000112513 0248_ $$2sideral$$a128230
000112513 037__ $$aART-2022-128230
000112513 041__ $$aeng
000112513 100__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, María
000112513 245__ $$aHigh-Performance Thin-Layer Chromatography-Densitometry-Tandem ESI-MS to Evaluate Phospholipid Content in Exosomes of Cancer Cells
000112513 260__ $$c2022
000112513 5060_ $$aAccess copy available to the general public$$fUnrestricted
000112513 5203_ $$aThe question of whether exosome lipids can be considered as potential cancer biomarkers faces our current limited knowledge of their composition. This is due to the difficulty in isolating pure exosomes, the variability of the biological sources from which they are extracted, and the uncertainty of the methods for lipid characterization. Here, we present a procedure to isolate exosomes and obtain a deep, repeatable, and rapid phospholipid (PL) composition of their lipid extracts, from embryonic murine fibroblasts (NIH-3T3 cell line) and none (B16-F1) and high (B16-F10) metastatic murine skin melanoma cells. The analytical method is based on High Performance Thin-Layer Chromatography with Ultraviolet and fluorescence densitometry and coupled to Electrospray (ESI)-tandem Mass Spectrometry (MS). Under the conditions described in this work, separation and determination of PL classes, (sphingomyelins, SM; phosphatidylcholines, PC; phosphatidylserines, PS; and phosphatidylethanolamines, PE) were achieved, expressed as mu g PL/100 mu g exosome protein, obtained by bicinchoninic acid assay (BCA). A detailed structural characterization of molecular species of each PL class was performed by simultaneous positive and negative ESI-MS and MS/MS directly from the chromatographic plate, thanks to an elution-based interface.
000112513 536__ $$9info:eu-repo/grantAgreement/ES/CSIC/2021-80E076$$9info:eu-repo/grantAgreement/ES/DGA/E25-20R$$9info:eu-repo/grantAgreement/EUR/ERC/ERC-AdG-742684$$9info:eu-repo/grantAgreement/ES/ISCIII-AES/PI21-00036$$9info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007
000112513 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000112513 590__ $$a5.6$$b2022
000112513 592__ $$a1.154$$b2022
000112513 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000112513 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000112513 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000112513 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000112513 593__ $$aComputer Science Applications$$c2022$$dQ1
000112513 593__ $$aInorganic Chemistry$$c2022$$dQ1
000112513 593__ $$aSpectroscopy$$c2022$$dQ1
000112513 593__ $$aOrganic Chemistry$$c2022$$dQ1
000112513 593__ $$aMolecular Biology$$c2022$$dQ2
000112513 593__ $$aCatalysis$$c2022$$dQ2
000112513 594__ $$a7.8$$b2022
000112513 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000112513 700__ $$aJarne, Carmen
000112513 700__ $$0(orcid)0000-0001-7299-0187$$aSaviróon, María
000112513 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, Pilar$$uUniversidad de Zaragoza
000112513 700__ $$aMembrado, Luis
000112513 700__ $$aCebolla, Vicente L.
000112513 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaría, Jesús$$uUniversidad de Zaragoza
000112513 7102_ $$11013$$2275$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Estomatología
000112513 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000112513 773__ $$g23, 3 (2022), 1150 [14 pp]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000112513 8564_ $$s1545487$$uhttps://zaguan.unizar.es/record/112513/files/texto_completo.pdf$$yVersión publicada
000112513 8564_ $$s2742479$$uhttps://zaguan.unizar.es/record/112513/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000112513 909CO $$ooai:zaguan.unizar.es:112513$$particulos$$pdriver
000112513 951__ $$a2024-03-18-13:22:12
000112513 980__ $$aARTICLE