000112765 001__ 112765 000112765 005__ 20240319080952.0 000112765 0247_ $$2doi$$a10.1093/jme/tjab148 000112765 0248_ $$2sideral$$a128383 000112765 037__ $$aART-2022-128383 000112765 041__ $$aeng 000112765 100__ $$0(orcid)0000-0001-5028-947X$$aCalvete, C. 000112765 245__ $$aExperimental study of the mechanical transmission of rabbit hemorrhagic disease virus (RHDV2/b) by Aedes Albopictus (Diptera: Clicidae) and Phlebotomus papatasi (diptera: psychodidae); 34447999 000112765 260__ $$c2022 000112765 5060_ $$aAccess copy available to the general public$$fUnrestricted 000112765 5203_ $$aRabbit hemorrhagic disease (RHD) is caused by a lagovirus mainly affecting European rabbits (Oryctolagus cuniculus), although other European and North American lagomorph species are also susceptible to fatal infection by the new viral variant RHDV2/b. In the present work, direct mechanical transmission of the rabbit hemorrhagic disease virus (RHDV2/b variant) by the hematophagous Diptera Aedes albopictus (Skuse) (Diptera: Culicidae) and the sand fly Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae) was tested. For each species, six and three laboratory rabbits were exposed to bites of dipterous females partially fed on RHDV2/b viral suspension 2 h and 24 h prior to exposure, respectively. The rabbits were then monitored for clinical changes and mortality for 35 d, and seroconversion was assessed by indirect ELISA. No rabbit died or showed clinical signs of disease, and seroconversion was recorded in two rabbits challenged with P. papatasi females fed the viral suspension 2 h prior to exposure. The number of RHDV2/b RNA copies/female was higher in Ae. albopictus than in P. papatasi but the decrease over time of RNA load in Ae. albopictus was greater than that in P. papatasi. The results of this study suggest the inability of Ae. albopictus to serve as a direct mechanical vector of RHDV2/b, but sand flies could play a role in the local transmission of RHD. © The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. 000112765 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A05-17R$$9info:eu-repo/grantAgreement/ES/DGA/A14-20R$$9info:eu-repo/grantAgreement/ES/INIA/E-RTA2014-00009-00-00 000112765 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000112765 590__ $$a2.1$$b2022 000112765 592__ $$a0.694$$b2022 000112765 591__ $$aVETERINARY SCIENCES$$b43 / 144 = 0.299$$c2022$$dQ2$$eT1 000112765 593__ $$aInsect Science$$c2022$$dQ1 000112765 591__ $$aENTOMOLOGY$$b30 / 100 = 0.3$$c2022$$dQ2$$eT1 000112765 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1 000112765 593__ $$aParasitology$$c2022$$dQ2 000112765 593__ $$aInfectious Diseases$$c2022$$dQ2 000112765 594__ $$a4.0$$b2022 000112765 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000112765 700__ $$0(orcid)0000-0001-7046-2997$$aDelacour, S.$$uUniversidad de Zaragoza 000112765 700__ $$aOropeza Velasquez, R. 000112765 700__ $$0(orcid)0000-0002-6279-0453$$aEstrada, R.$$uUniversidad de Zaragoza 000112765 700__ $$aSarto, M.P. 000112765 700__ $$0(orcid)0000-0001-6042-3134$$aIguacel, L. 000112765 700__ $$0(orcid)0000-0003-0663-8411$$aLucientes, J .$$uUniversidad de Zaragoza 000112765 700__ $$aCalvo, J.H. 000112765 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000112765 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cProy. investigación HRA 000112765 773__ $$g59, 1 (2022), 350-354$$pJ. med. entomol.$$tJournal of medical entomology$$x0022-2585 000112765 8564_ $$s215470$$uhttps://zaguan.unizar.es/record/112765/files/texto_completo.pdf$$yVersión publicada 000112765 8564_ $$s2471824$$uhttps://zaguan.unizar.es/record/112765/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000112765 909CO $$ooai:zaguan.unizar.es:112765$$particulos$$pdriver 000112765 951__ $$a2024-03-18-13:10:39 000112765 980__ $$aARTICLE