000117138 001__ 117138
000117138 005__ 20240319080952.0
000117138 0247_ $$2doi$$a10.1016/j.prevetmed.2022.105612
000117138 0248_ $$2sideral$$a128441
000117138 037__ $$aART-2022-128441
000117138 041__ $$aeng
000117138 100__ $$aFerreras-Colino, Elisa
000117138 245__ $$aIs serology a realistic approach for monitoring red deer tuberculosis in the field?
000117138 260__ $$c2022
000117138 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117138 5203_ $$aTuberculosis (TB) is a zoonotic mycobacterial infection with great importance in human health, animal production, and wildlife conservation. Although an ambitious eradication programme in cattle has been implemented for decades, TB-free status has not yet been achieved in most of Spain, where animal TB persists in a multi-host system of domestic and wild hosts, including the red deer (Cervus elaphus). However, information on long time series and trends of TB prevalence in wildlife is scarce. The diagnosis of TB in wild red deer is often based on gross pathology and bacteriological culture confirmation, although recently serological assays have been developed to detect anti- Mycobacterium tuberculosis Complex (MTC) antibodies. Particularly, protein complex P22 has demonstrated to yield good specificity and sensitivity in the serological diagnosis of MTC for red deer, as well as cattle, goats, sheep, pigs, wild boar, and European badger. Thus, the objective of the present study was to compare the performance of the P22-ELISA with TB-compatible lesion detection, as well as to assess the potential application of each technique for determining spatiotemporal trends and risk factors of MTC infection in wild red deer from low and high TB prevalence areas of Spain over the last two decades. We tested 5095 sera from 13 wild populations by indirect ELISA using P22 as antigen. Mean seroprevalence (13.22%, CI95: 12.32–14.18) was compared with the prevalence of macroscopic TB-compatible lesions (6.94%, CI95: 6.18–7.79). The results evidenced a poor agreement between both techniques (K < 0.3), although generalized TB-lesions and anti-P22 antibodies showed a positive association (¿² = 9.054, P = 0.004). Consequently, TB-lesion based prevalence and seroprevalence cannot be considered as equivalent for TB surveillance in red deer. Regarding the spatiotemporal trend of TB in red deer in Spain, we observed a North-South gradient of TB occurrence North: 1.23% (CI95: 0.77–1.97) of TB-lesions and 12.55% (CI95: 10.91–14.41) of P22-ELISA; Centre: 7.10% (CI95: 6.04–8.33) and 8.74% (CI95: 7.57–10.08); South: 21.04% (CI95:17.81–24.69) and 23.09% (CI95: 19.73–26.84), respectively]. Overall, there was a stability over time, with higher prevalence in adults belonging to densely populated sites. We conclude that the P22-ELISA alone is not sufficiently reliable for TB surveillance in red deer at large spatiotemporal scales. Instead, we recommend combining gross pathology and P22-ELISA.
000117138 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CGL2017-89866
000117138 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117138 590__ $$a2.6$$b2022
000117138 592__ $$a0.708$$b2022
000117138 591__ $$aVETERINARY SCIENCES$$b21 / 144 = 0.146$$c2022$$dQ1$$eT1
000117138 593__ $$aFood Animals$$c2022$$dQ1
000117138 593__ $$aAnimal Science and Zoology$$c2022$$dQ1
000117138 594__ $$a5.4$$b2022
000117138 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117138 700__ $$aMoreno, Inmaculada
000117138 700__ $$0(orcid)0000-0002-6770-3400$$aArnal, María Cruz$$uUniversidad de Zaragoza
000117138 700__ $$aBalseiro, Ana
000117138 700__ $$aAcevedo, Pelayo
000117138 700__ $$aDomínguez, Mercedes
000117138 700__ $$0(orcid)0000-0003-3289-4267$$aFernández de Luco, Daniel$$uUniversidad de Zaragoza
000117138 700__ $$aGortázar, Christian
000117138 700__ $$aRisalde, María A.
000117138 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000117138 773__ $$g202 (2022), 105612 [9 pp.]$$pPrev. vet. med.$$tPREVENTIVE VETERINARY MEDICINE$$x0167-5877
000117138 8564_ $$s1707267$$uhttps://zaguan.unizar.es/record/117138/files/texto_completo.pdf$$yVersión publicada
000117138 8564_ $$s2193824$$uhttps://zaguan.unizar.es/record/117138/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117138 909CO $$ooai:zaguan.unizar.es:117138$$particulos$$pdriver
000117138 951__ $$a2024-03-18-13:14:17
000117138 980__ $$aARTICLE