000117163 001__ 117163
000117163 005__ 20240319080958.0
000117163 0247_ $$2doi$$a10.1186/s12989-022-00460-3
000117163 0248_ $$2sideral$$a128419
000117163 037__ $$aART-2022-128419
000117163 041__ $$aeng
000117163 100__ $$aAimonen, Kukka
000117163 245__ $$aSurface functionalization and size modulate the formation of reactive oxygen species and genotoxic effects of cellulose nanofibrils; 35296350
000117163 260__ $$c2022
000117163 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117163 5203_ $$aBackground: Cellulose nanofibrils (CNFs) have emerged as a sustainable and environmentally friendly option for a broad range of applications. The fibrous nature and high biopersistence of CNFs call for a thorough toxicity assessment, but it is presently unclear which physico-chemical properties could play a role in determining the potential toxic response to CNF. Here, we assessed whether surface composition and size could modulate the genotoxicity of CNFs in human bronchial epithelial BEAS-2B cells. We examined three size fractions (fine, medium and coarse) of four CNFs with different surface chemistry: unmodified (U-CNF) and functionalized with 2, 2, 6, 6-tetramethyl-piperidin-1-oxyl (TEMPO) (T-CNF), carboxymethyl (C-CNF) and epoxypropyltrimethylammonium chloride (EPTMAC) (E-CNF). In addition, the source fibre was also evaluated as a non-nanosized material. Results: The presence of the surface charged groups in the functionalized CNF samples resulted in higher amounts of individual nanofibrils and less aggregation compared with the U-CNF. T-CNF was the most homogenous, in agreement with its high surface group density. However, the colloidal stability of all the CNF samples dropped when dispersed in cell culture medium, especially in the case of T-CNF. CNF was internalized by a minority of BEAS-2B cells. No remarkable cytotoxic effects were induced by any of the cellulosic materials. All cellulosic materials, except the medium fraction of U-CNF, induced a dose-dependent intracellular formation of reactive oxygen species (ROS). The fine fraction of E-CNF, which induced DNA damage (measured by the comet assay) and chromosome damage (measured by the micronucleus assay), and the coarse fraction of C-CNF, which produced chromosome damage, also showed the most effective induction of ROS in their respective size fractions. Conclusions: Surface chemistry and size modulate the in vitro intracellular ROS formation and the induction of genotoxic effects by fibrillated celluloses. One cationic (fine E-CNF) and one anionic (coarse C-CNF) CNF showed primary genotoxic effects, possibly partly through ROS generation. However, the conclusions cannot be generalized to all types of CNFs, as the synthesis process and the dispersion method used for testing affect their physico-chemical properties and, hence, their toxic effects.
000117163 536__ $$9info:eu-repo/grantAgreement/EC/H2020/788489/EU/Bioproducts Engineered from Lignocelluloses: from plants and upcycling to next generation materials/BioELCell$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 788489-BioELCell
000117163 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117163 590__ $$a10.0$$b2022
000117163 592__ $$a1.805$$b2022
000117163 591__ $$aTOXICOLOGY$$b4 / 94 = 0.043$$c2022$$dQ1$$eT1
000117163 593__ $$aHealth, Toxicology and Mutagenesis$$c2022$$dQ1
000117163 593__ $$aToxicology$$c2022$$dQ1
000117163 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000117163 594__ $$a13.5$$b2022
000117163 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117163 700__ $$aImani, Monireh
000117163 700__ $$aHartikainen, Mira
000117163 700__ $$aSuhonen, Satu
000117163 700__ $$aVanhala, Esa
000117163 700__ $$0(orcid)0000-0002-1946-1187$$aMoreno, Carlos$$uUniversidad de Zaragoza
000117163 700__ $$aRojas, Orlando J.
000117163 700__ $$aNorppa, Hannu
000117163 700__ $$0(orcid)0000-0003-2936-242X$$aCatalán, Julia$$uUniversidad de Zaragoza
000117163 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000117163 773__ $$g19 (2022), 19 [21 pp.]$$pPart fibre toxicol.$$tPARTICLE AND FIBRE TOXICOLOGY$$x1743-8977
000117163 8564_ $$s3620217$$uhttps://zaguan.unizar.es/record/117163/files/texto_completo.pdf$$yVersión publicada
000117163 8564_ $$s2529426$$uhttps://zaguan.unizar.es/record/117163/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117163 909CO $$ooai:zaguan.unizar.es:117163$$particulos$$pdriver
000117163 951__ $$a2024-03-18-13:49:14
000117163 980__ $$aARTICLE