117168 20240319080958.0 doi 10.1016/j.jcis.2022.03.036 sideral 128425 ART-2022-128425 eng Bonet-Aleta, Javier Universidad de Zaragoza (orcid)0000-0002-1791-0188 Glutathione-Triggered catalytic response of Copper-Iron mixed oxide Nanoparticles. Leveraging tumor microenvironment conditions for chemodynamic therapy 2022 Access copy available to the general public Unrestricted Heterogeneous catalysis has emerged as a promising alternative for the development of new cancer therapies. In addition, regarding the tumor microenvironment as a reactor with very specific chemical features has provided a new perspective in the search for catalytic nanoarchitectures with specific action against chemical species playing a key role in tumor metabolism. One of these species is glutathione (GSH), whose depletion is the cornerstone of emerging strategies in oncology, since this metabolite plays a pivotal regulatory role as antioxidant agent, dampening the harmful effects of intracellular reactive oxidative species (ROS). Herein, we present copper-iron oxide spinel nanoparticles that exhibit a versatile and selective catalytic response to reduce GSH levels while generating ROS in a cascade reaction. We demonstrate a clear correlation between GSH depletion and apoptotic cell death in tumor cells in the presence of the copper-iron nanocatalyst. Furthermore, we also provide a novel analytical protocol, alternative to state-of-the-art commercial kits, to accurately monitoring the concentration of GSH intracellular levels in both tumor and healthy cells. We observe a selective action of the nanoparticles, with lower toxicity in healthy cell lines, whose intrinsic GSH levels are lower, and intense apoptosis in tumor cells accompanied by a fast reduction of GSH levels. info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 9.9 2022 CHEMISTRY, PHYSICAL 29 / 161 = 0.18 2022 Q1 T1 1.604 2022 Biomaterials 2022 Q1 Surfaces, Coatings and Films 2022 Q1 Electronic, Optical and Magnetic Materials 2022 Q1 Colloid and Surface Chemistry 2022 Q1 15.5 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sancho-Albero, Maria (orcid)0000-0001-8762-5457 Calzada-Funes, Javier Universidad de Zaragoza (orcid)0000-0002-1603-7305 Irusta, Silvia Universidad de Zaragoza (orcid)0000-0002-2966-9088 Martin-Duque, Pilar Universidad de Zaragoza (orcid)0000-0003-4160-9720 Hueso, Jose L. Universidad de Zaragoza (orcid)0000-0002-4546-4111 Santamaria, Jesus Universidad de Zaragoza (orcid)0000-0002-8701-9745 1013 275 Universidad de Zaragoza Dpto. Cirugía Área Estomatología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 617 (2022), 704-717 J. colloid interface sci. Journal of Colloid and Interface Science 0021-9797 3425963 http://zaguan.unizar.es/record/117168/files/texto_completo.pdf Versión publicada 1879516 http://zaguan.unizar.es/record/117168/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117168 articulos driver 2024-03-18-13:53:34 ARTICLE