000117172 001__ 117172
000117172 005__ 20240319080959.0
000117172 0247_ $$2doi$$a10.3390/antiox11030581
000117172 0248_ $$2sideral$$a128626
000117172 037__ $$aART-2022-128626
000117172 041__ $$aeng
000117172 100__ $$aBidooki, S. H.
000117172 245__ $$aSqualene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way
000117172 260__ $$c2022
000117172 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117172 5203_ $$aVirgin olive oil, the main source of fat in the Mediterranean diet, contains a substantial amount of squalene which possesses natural antioxidant properties. Due to its highly hydrophobic nature, its bioavailability is reduced. In order to increase its delivery and potentiate its actions, squalene has been loaded into PLGA nanoparticles (NPs). The characterization of the resulting nanoparticles was assessed by electron microscopy, dynamic light scattering, zeta potential and highperformance liquid chromatography. Reactive oxygen species (ROS) generation and cell viability assays were carried out in AML12 (alpha mouse liver cell line) and a TXNDC5-deficient AML12 cell line (KO), which was generated by CRISPR/cas9 technology. According to the results, squalene was successfully encapsulated in PLGA NPs, and had rapid and efficient cellular uptake at 30 µM squalene concentration. Squalene reduced ROS in AML12, whereas ROS levels increased in KO cells and improved cell viability in both when subjected to oxidative stress by significant induction of Gpx4. Squalene enhanced cell viability in ER-induced stress by decreasing Ern1 or Eif2ak3 expressions. In conclusion, TXNDC5 shows a crucial role in regulating ER-induced stress through different signaling pathways, and squalene protects mouse hepatocytes from oxidative and endoplasmic reticulum stresses by several molecular mechanisms depending on TXNDC5. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
000117172 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/B16-20R$$9info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00
000117172 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117172 590__ $$a7.0$$b2022
000117172 592__ $$a1.084$$b2022
000117172 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b46 / 285 = 0.161$$c2022$$dQ1$$eT1
000117172 593__ $$aBiochemistry$$c2022$$dQ1
000117172 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b13 / 142 = 0.092$$c2022$$dQ1$$eT1
000117172 593__ $$aClinical Biochemistry$$c2022$$dQ1
000117172 591__ $$aCHEMISTRY, MEDICINAL$$b6 / 60 = 0.1$$c2022$$dQ1$$eT1
000117172 593__ $$aFood Science$$c2022$$dQ1
000117172 593__ $$aPhysiology$$c2022$$dQ1
000117172 593__ $$aMolecular Biology$$c2022$$dQ2
000117172 593__ $$aCell Biology$$c2022$$dQ2
000117172 594__ $$a8.8$$b2022
000117172 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117172 700__ $$0(orcid)0000-0002-9324-0446$$aAlejo, T.$$uUniversidad de Zaragoza
000117172 700__ $$0(orcid)0000-0002-9023-741X$$aSánchez-Marco, J.
000117172 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R.$$uUniversidad de Zaragoza
000117172 700__ $$aAbuobeid, R.
000117172 700__ $$aBurillo, J. C.
000117172 700__ $$aLasheras, R.
000117172 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, V.$$uUniversidad de Zaragoza
000117172 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, M. J.$$uUniversidad de Zaragoza
000117172 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, M.$$uUniversidad de Zaragoza
000117172 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza
000117172 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000117172 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000117172 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000117172 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000117172 773__ $$g11, 3 (2022), 581 [22 pp]$$pAntioxidants$$tAntioxidants$$x2076-3921
000117172 8564_ $$s5010705$$uhttps://zaguan.unizar.es/record/117172/files/texto_completo.pdf$$yVersión publicada
000117172 8564_ $$s2818797$$uhttps://zaguan.unizar.es/record/117172/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117172 909CO $$ooai:zaguan.unizar.es:117172$$particulos$$pdriver
000117172 951__ $$a2024-03-18-13:56:23
000117172 980__ $$aARTICLE